Method of inhibiting dyrk1b

ABSTRACT

The present invention relates to a DYRK1B inhibitor for use in the treatment of cancer, wherein in said cancer and/or in cells of said cancer the hedgehog signaling pathway is activated, and in particular the activation of the hedgehog signaling pathway is independent of signaling by the G protein-coupled receptor Smoothened.

SEQUENCE LISTING

The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Jun. 17, 2014, is named BOEHMERP-0207_SL.txt and is 1,746 bytes in size.

BACKGROUND OF THE INVENTION

The Dual-Specificity-Tyrosine-Regulated Kinase 1B (DYRK1B, also known as Minibrain-Regulated Kinase MIRK) belongs to the DYRK family of serine/threonine kinases, which, based on sequence and structural homologies, can be divided into three subgroups: the YAK group with no members in the animal kingdom, the DYRK1 and the DYRK2 subgroup. The two DYRK1 subgroup members DYRK1A and DYRK1B share 85% identity at the amino acid level, though expression and functional characteristics are distinct (Aranda et al., 2011).

The human DYRK1B gene encodes a 69 kDa protein with 629 amino acids in length. Alternative splicing and differential promoter engagement can yield two additional, slightly shorter DYRK1B isoforms with differential expression patterns, the shorter of which lacking kinase activity (Leder et al., 2003).

The regulation of DYRK1B catalytic activity and function is not entirely understood. Given the extensive sequence similarity to DYRK1A, the intrinsic regulatory and catalytic properties of DYRK1B can, to a certain extent, be inferred from studies of DYRK1A regulation. DYRK family members are arginine-directed serine/threonine kinases, with DYRK1B phosphorylating either serine in the consensus substrate sequence SPSxxR (Friedman, 2007; Himpel et al., 2000). Activation of DYRK1B involves an intramolecular tyrosine (Y) auto-phosphorylation of the second tyrosine of an Y×Y motif in the conserved kinase domain and activation loop, respectively (Becker and Sippl, 2011; Himpel et al., 2000; Nolen et al., 2004). Notably, this Y-phosphorylation-dependent activation step occurs only during translation of DYRK kinases, resulting in activated DYRK proteins with Ser/Thr kinase activity (Lochhead et al., 2005). This implies that additional processes including protein-protein interactions, further post-translational modifications and/or subcellular localization control the response of DYRK kinases to extracellular signals. For instance, signaling via RAS/RAC/MKK3 is able to stimulate DYRK1B activity in certain cellular contexts such as in pancreatic cancer cells and interaction of DYRK1A with 14-3-3 proteins significantly enhances DYRK kinase activity (Deng et al., 2003; Jin et al., 2005; Jin et al., 2007; Kim et al., 2004; Lim et al., 2002).

During normal development DYRK1B expression is preferentially restricted to testes and muscle cells (Leder et al., 2003; Leder et al., 1999). In muscle its expression is regulated by RHO and basic Helix-Loop-Helix transcription factors via binding to an E-box in the DYRK1B promoter (Deng et al., 2003; Friedman, 2007). Inhibition and overexpression studies suggest a pro-differentiation activity of DYRK1B in myoblast differentiation by enhancing the expression of myogenic transcription factors such as Mef2 (Deng et al., 2005). This effect is mediated by DYRK1B-dependent phosphorylation of class II histone deacetylases (HDAC) thereby freeing Mef2 from complexes with inhibitory HDACs and allowing Mef2 to exert its pro-myogenic function (Deng et al., 2005). In addition to its role in myoblast differentiation, DYRK1B also controls cell cycle arrest by phosphorylation-dependent destabilization of D-type cyclins and stabilization of cell cycle inhibitors including p27 and p21 (Deng et al., 2004; Ewton et al., 2011; Mercer et al., 2005; Zou et al., 2004). Like DYRK1A, for which a larger number of substrate proteins has already been identified, DYRK1B can act as co-activator of the FOXO1a transcription factor, thereby regulating glucose-6-phosphatase expression (von Groote-Bidlingmaier et al., 2003).

DYRK1B deficient mice do not display any evident developmental defects and survive several weeks post birth (Leder et al., 2003). Details of the DYRK1B mutant phenotype remain to be reported. By contrast, DYRK1A deficiency in mice results in an embryonic lethal phenotype (Fotaki et al., 2002).

DYRK1B in Cancer

Several recent studies have implicated DYRK1B as a putative oncogenic factor in different cancer entities. DYRK1B localizes to the chromosomal region 19q13, which is frequently amplified in pancreatic and ovarian cancers (Friedman, 2007; Karhu et al., Genes Chromosomes Cancer. 2006 August; 45(8):721-730; Lee et al., 2000). Accordingly, DYRK1B is strongly expressed in a fraction of pancreatic and ovarian cancer cell lines (Friedman, 2007; Hu and Friedman, 2010). Notably, in pancreatic cancer DYRK1B acts as survival effector kinase downstream of RAS-RAC1 to promote viability and clonal growth of cancer cells (Jin et al., 2007). In addition, several in vitro studies suggest that DYRK1B has pro-oncogenic function in colon cancer, osteosarcoma and rhabdomyosarcoma (RMS). RNA interference and overexpression studies demonstrated a pro-survival role of DYRK1B in colon cancer, osteosarcoma, RMS and pancreatic cancer (Deng et al., 2009; Deng et al., 2006; Friedman, 2011; Jin et al., 2007; Mercer et al., 2006; Yang et al., Carcinogenesis. 2010 April; 31(4):552-558). The pro-survival activity in sarcoma can—at least in part—be ascribed to the role of DYRK1B in promoting the inactivation of reactive oxygen species (ROS). DYRK1B is able to increase the expression of ROS detoxifying enzymes including superoxide dismutases 2 and 3 (Deng et al., 2009; Hu and Friedman, 2010). This may also explain the enhanced sensitivity of DYRK1B-depleted cancer cells to certain chemotherapeutic drugs such as cisplatin known to increase toxic ROS levels (Hu and Friedman, 2010). Recently published results indicate that meningioma, in particular Non-NF2 Meningioma, may also depend on DYRK1B activity e.g. due to mutations in factors such as TRAF7, KLF4, AKT1, and/or SMO (Clark, V. E. et al., Science, 2013 Jan. 24, Epub ahead of print, PMID (PubMed-ID) 23348505—as supplied by publisher; Aavikko M. et al., Am J Hum Genet, 2012 Sep. 7, 91(3), 520-526; Kijima C. et al., Fam Cancer. 2012 Dec. 11(4), 565-570).

Hedgehog Signaling in Cancer Therapy

The Hedgehog (HH)/GLI signal transduction pathway is a key regulator of multiple developmental processes. Uncontrolled activation of HH/GLI signaling is a common feature of many human malignancies including cancers of the brain, skin, gastro-intestinal tract, prostate, breast lung, muscle and bone (reviewed in (Beachy et al., 2004a, b; Epstein, 2008; Kasper et al., 2012; Kasper et al., 2006; Merchant and Matsui, 2010; Ng and Curran, 2011; Ruiz i Altaba et al., 2007; Ruiz i Altaba et al., 2002; Scales and de Sauvage, 2009; Teglund and Toftgard, 2010; Theunissen and de Sauvage, 2009).

Precise reversible regulation of Hedgehog signaling is a complex process and mandatory for proper normal development of invertebrate and vertebrate organisms (for detailed reviews see (Huangfu and Anderson, 2006; Ingham and McMahon, 2001; Teglund and Toftgard, 2010)). In the absence of HH ligand, HH signaling is repressed by the activity of the HH receptor Patched (PTCH), a twelve-transmembrane domain protein whose intracellular localization is concentrated at the base of the primary cilium, a single antenna-like cell surface compartment that coordinates HH signal transduction. Unliganded PTCH prevents the translocation of the G-protein coupled receptor-like protein and essential pathway effector Smoothened into the primary cilium (Corbit et al., 2005; Rohatgi et al., 2007; Rohatgi and Scott, 2007). This leads to proteolytic cleavage of the latent zinc finger transcription factors GLI3—and to some extent also of GLI2—into C-terminally truncated repressor forms (GLI^(R)). GLI^(R) formation involves preceding and sequential phosphorylation by protein kinase A (PKA), glycogen synthase kinase 3-beta (GSK) and casein kinase I (CKI) (Price and Kalderon, 2002) as well as a functional primary cilium (Smith and Rohatgi, 2011; Wang et al., 2000; Wen et al., 2010; Wong et al., 2009). Following processing, GLI^(R) translocates to the nucleus to bind to HH target gene promoters and repress target gene expression (Aza-Blanc and Kornberg, 1999; Aza-Blanc et al., 1997). GLI signals are also negatively regulated by proteasome-mediated degradation of GLI and by binding to Suppressor of Fused (SUFU), which sequesters GLI proteins in the cytoplasm and also contributes to GLI processing in the primary cilium (Humke et al., 2010; Kogerman et al., 1999).

The therapeutic relevance of targeting HH/GLI signaling in human cancers with genetic, ligand-independent activation of HH/GLI signaling has recently been demonstrated for BCC and medulloblastoma. In both malignant entities, inhibition of the essential HH pathway effector Smoothened had a dramatic therapeutic benefit (Rudin et al., 2009; Skvara et al., 2011; Von Hoff et al., 2009). Whether Smoothened antagonists will display therapeutic efficacy in HH ligand dependent cancers remains to be shown. Ongoing clinical trials with Smoothened antagonists from different pharmaceutical companies will eventually answer the question of the clinical efficacy of targeting Smoothened in Hedgehog associated malignancies (Aberger et al., 2012; Lin and Matsui, 2012; Ng and Curran, 2011; Scales and de Sauvage, 2009). Clinical studies with small molecule Smoothened inhibitors to treat patients with metastatic colorectal cancer, ovarian cancer or pancreatic cancer failed to demonstrate therapeutic efficacy of Smoothened antagonists in combination with currents treatment regimens (Ng and Curran, 2011). One of the reasons for the lack of therapeutic efficacy of Smoothened inhibitors may be explained by Smoothened-independent activation of GLI transcription factors in different cancer entities such as pancreatic cancer, melanoma or Ewing's sarcoma. This non-canonical activation of GLI transcription factors can be induced by a variety of signals frequently hyperactive in malignant cells including TGF-b/SMAD, RAS-MEK/ERK, PI3K/AKT, EGFR signaling or the EWS-FLI1 oncogene (reviewed in Aberger et al., 2012; Mangelberger et al., 2012; Stecca and Ruiz, 2010).

Regulation of HH/GLI Signaling by DYRK Family Members

The first regulatory interactions between DYRK family members and the HH/GLI pathway came from studies of DYRK1A and its impact on the transcriptional activity of the GLI zinc finger transcription factors mediating the transcriptional output of HH pathway activation. Using reporter gene based assays, Mao et al. have shown that DYRK1A is able to enhance the activity of the GLI1 activator and stimulate HH target gene expression, respectively. DYRK1A can phosphorylate GLI1 in vitro and enhance the nuclear level of GLI1. Direct modification of GLI1 and enhanced nuclear localization in response to DYRK1A activity are likely to account for the enhanced expression of HH target genes (Mao et al., 2002).

While DYRK1A enhances GLI activity, the class II DYRK family member DYRK2 acts as negative regulator of GLI activity. DYRK2 can directly phosphorylate GLI2 and GLI3 resulting in destabilization of GLI2/3 and enhanced proteasome-dependent degradation.

Mutation of the DYRK2 substrate phosphorylation sites S384 and S1011 in GLI2 rendered GLI2 resistant to DYRK2 mediated inhibition of transcriptional activity and proteasomal degradation (Varjosalo et al., 2008).

Analysis of DYRK1B function in HH-unresponsive RAS mutant pancreatic cancer cells revealed another regulatory mechanism by which DYRK kinases can affect the activity of HH signaling. Lauth et al. (2010) provide evidence that DYRK1B is involved in an autocrine-to-paracrine shift of HH signaling triggered by mutant RAS. This study suggests that oncogenic RAS signaling in pancreatic cancer cells increases HH ligand expression though at the same time it also prevents autocrine HH pathway activation (Lauth et al., 2010). RAS signaling therefore contributes to paracrine HH signaling, with tumor cells representing the signal source and adjacent stroma cells the signal-receiving compartment (Yauch et al., 2008). Like RAS, expression of the RAS effector DYRK1B in HH activated mouse fibroblasts inhibited HH signaling, suggesting that DYRK1B can act downstream of RAS to prevent autocrine HH signaling. Further, RNAi knockdown of RAS and DYRK1B in RAS mutant pancreatic cancer cells both led to a GLI2-dependent increase in GLI1 mRNA expression (Lauth et al., 2010). The detailed mechanisms of HH pathway inhibition by DYRK1B remain unknown.

Together, these reports suggest that DYRK2 and DYRK1B can have a repressive effect on HH/GLI signaling while DYRK1A functions as positive regulator of GLI transcriptional activity.

SMO targeting is a valid approach for the treatment of cancer entities involving canonical Hedgehog signaling such as BCC and medulloblastoma. However, rapid resistance development to SMO inhibitors as well as non-canonical SMO-independent activation of oncogenic GLI (e.g. in pancreatic cancer, esophageal carcinoma and Ewing's sarcoma) confers severe constraints to the therapeutic application of SMO inhibitors. Further, multiple oncogenic signals including RTK, MAPK and PI3K pathways promote oncogenic GLI function independent of SMO. Thus, there is a high medical need for the identification of drug targets downstream of SMO (and therefore independent of SMO activation) for the rational design of GLI intervention strategies. This would open up the opportunity for the design of novel anti-GLI drugs with a clear medical benefit for the treatment of SMO-dependent and the many SMO-independent or SMO-inhibitor resistant cancers.

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BRIEF SUMMARY OF THE INVENTION

The various and partially opposing functions of DYRK kinases reported previously prompted the present inventors to carefully study the specific involvement of said kinases in oncogenic HH signaling, with a focus on DYRK1 kinases. The impact of DYRK1A and DYRK1B on HH signal activity was re-addressed using an array of cellular in vitro and in vivo test systems including SMO-dependent and SMO-independent model systems from different species.

The present invention relates to a method of treating cancer in a patient suffering from cancer, said method comprising administering a therapeutically effective amount of a DYRK1B inhibitor to said patient.

The present invention further relates to a DYRK1B inhibitor for use in the treatment of cancer.

The present invention further relates to the use of a DYRK1B inhibitor for producing a medicament for the treatment of cancer.

The present invention further relates to a pharmaceutical combination comprising a DYRK1B inhibitor, in particular for use in the treatment of cancer.

The present invention further relates to a cancer medicament comprising a DYRK1B inhibitor.

The present invention further relates to a method of inhibiting DYRK1B in vitro, a method of inhibiting DYRK1B in a mammal, a method of inhibiting the hedgehog signaling pathway in a mammal, and a method of preventing the formation of resistance of cancer cells against chemotherapeutic agents in a patient suffering from cancer, all of which are detailed further herein below.

DETAILED DESCRIPTION OF THE INVENTION

In certain embodiments, the present invention relates to the matter enumerated in the following items:

-   1. A DYRK1B inhibitor for use in the treatment of cancer, wherein in     said cancer and/or in cells of said cancer the hedgehog signaling     pathway is activated. -   2. The DYRK1B inhibitor according to item 1, wherein said cancer     and/or cells of said cancer do not respond to Smoothened inhibitor     therapy. -   3. The DYRK1B inhibitor according to any of items 1 or 2, wherein in     said cancer and/or in cells of said cancer the activation of the     hedgehog signaling pathway is independent of signaling by the G     protein-coupled receptor Smoothened. -   4. The DYRK1B inhibitor according to any of items 1 to 3, wherein     said cancer is not responsive to inhibition of the G protein-coupled     receptor Smoothened. -   5. The DYRK1B inhibitor according to any of items 1 to 4, wherein in     said cancer and/or in cells of said cancer the G protein-coupled     receptor Smoothened is not responsive to inhibition by Smoothened     inhibitors. -   6. The DYRK1B inhibitor according to any of items 1 to 5, wherein     said cancer is selected from the group comprising cancer of the     breast, esophagus, gastrointestinal tract, gastro-intestinal stromal     tumors, pancreas, prostate, biliary tract, bladder, basal cell     carcinoma, medulloblastoma, rhabdomyosarcoma, glioma, small-cell     lung cancer, oral squamous cell carcinoma, melanoma, colorectal     cancer, non-small cell lung cancer, osteosarcoma, glioblastoma,     chronic lymphacytic leukemia, chronic myeloid leukemia, multiple     myeloma, acute myeloid leukemia, ovarian cancer, meningioma,     particularly Non-NF2 Meningiomas, and liver cancer. -   7. The DYRK1B inhibitor of any of items 1 to 6, wherein said DYRK1B     inhibitor is a small molecule. -   8. The DYRK1B inhibitor of any of items 1 to 7, wherein said DYRK1B     inhibitor is a compound of the below formula (I):

In the method of item 1, said method can in certain embodiments comprise inhibiting the expression of a gene encoding for DYRK1B, wherein more particularly the expression of said gene is inhibited on the DNA level and/or on the RNA level.

-   9. The DYRK1B inhibitor of any of items 1 to 10, wherein said     patient is a human. -   10. The DYRK1B inhibitor of any of items 1 to 11, wherein cancer     stem cells are inhibited in said cancer. -   11. A method of inhibiting DYRK1B in vitro, said method comprising     adding a DYRK1B inhibitor to a sample comprising cancer cells     wherein the hedgehog signaling pathway is activated.     -   In certain embodiments the DYRK1B inhibitor is added in an         amount which is effective to inhibit DYRK1B by at least 50%,         more particularly at least 75%, even more particularly at least         90% in said sample. This can be determined via determining the         expression level of a target gene of the Hedgehog signaling         pathway via the methods described herein, e.g. qPCR or Western         Blot. -   12. A DYRK1B inhibitor for use in therapy for preventing the     formation of resistance of cancer cells against chemotherapeutic     agents in a patient suffering from cancer, wherein in said cancer     and/or in cells of said cancer the hedgehog signaling pathway is     activated.

In principle, in the embodiments of the present invention, the DYRK1B inhibitor can be any molecule which inhibits DYRK1B. In certain embodiments of the present invention, the DYRK1B inhibitor is selected from the group comprising the compound of the above formula (I), harmine, and the shRNA targeting DYRK1B (shDYRK1B) as described herein, particularly the compound of the above formula (I).

In particular embodiments of the present invention, in said cancer, the hedgehog signaling pathway is activated.

In particular embodiments of the present invention, in cells of said cancer, the hedgehog signaling pathway is activated.

In further particular embodiments of the present invention, said cancer does not respond to Smoothened inhibitor therapy.

In further particular embodiments of the present invention, cells of said cancer do not respond to Smoothened inhibitor therapy.

In further particular embodiments of the present invention, in said cancer the activation of the hedgehog signaling pathway is independent of signaling by the G protein-coupled receptor Smoothened.

In further particular embodiments of the present invention, in cells of said cancer the activation of the hedgehog signaling pathway is independent of signaling by the G protein-coupled receptor Smoothened.

In further particular embodiments of the present invention, in said cancer the G protein-coupled receptor Smoothened is not responsive to inhibition by Smoothened inhibitors.

In further particular embodiments of the present invention, in cells of said cancer the G protein-coupled receptor Smoothened is not responsive to inhibition by Smoothened inhibitors.

In the context of the present invention, said cancer or cells of said cancer being not responsive to Smoothened inhibitor therapy includes both the case that the activation of the hedgehog signaling pathway is independent of signaling by the G protein-coupled receptor Smoothened, and that the G protein-coupled receptor Smoothened is not responsive to inhibition by Smoothened inhibitors.

In the present invention patients wherein in said cancer, or in cells of said cancer, the hedgehog signaling pathway is activated are in short referred to as “Hedgehog dependent patients”, and patients wherein in said cancer, or in the cells of said cancer, the hedgehog signaling pathway is not activated are in short referred to as “Hedgehog independent patients”. In the present invention, patients e.g. can be stratified into Hedgehog dependent patients and Hedgehog independent patients by a procedure comprising the steps of 1) providing a sample from said patient, wherein said sample comprises cancer cells from said patient,

-   2) optionally subjecting said sample to a work-up step, -   3) adding a labeled antibody which specifically binds to at least     one protein playing a role in the hedgehog signaling pathway,     -   or     -   adding a first antibody which specifically binds to at least one         protein playing a role in the hedgehog signaling pathway, and         subsequently adding a second antibody which specifically binds         to said first antibody, and wherein said second antibody is a         labeled antibody, -   4) washing said sample after step 3, -   5) determining whether said labeled antibody is detectable in said     sample after step 4), -   6) if in step 5) said marker moiety is detectable, classifying said     patient as Hedgehog dependent patient, and if in step 5) said marker     moiety is not detectable, classifying said patient as Hedgehog     independent patient.

Antibodies used in the present invention are typically monoclonal antibodies.

The label in said labeled antibody can be selected from any label typically used as antibody label in the field of biochemistry, cellular biology, immunochemistry, etc., for a label selected from the group comprising a fluorescence label, a dye, a FRET label, a radioactive label. moiety, or an enzymatically active moiety. Said enzymatically active moiety can process a reaction which in turn results in the release of a detectable substance, e.g. a dye.

In the above method of stratifying patients into Hedgehog dependent patients and Hedgehog independent patients, the work-up step is e.g. in particular embodiments selected from the group comprising preservation, embedding, slicing and staining. Preservation can be performed by cryopreservation or fixation by e.g. formaldehyde or ethanol. Embedding the tumor material prepares it for slicing. Staining can be performed with direct or indirect methods. For further information and examples see DOI: 10.1354/vp.42-4-405 J. A. Ramos-Vara, Technical Aspects of Immunohistochemistry, (2005) 42: 405 Vet Pathol.

In the context of the present invention, the expression “said labeled antibody is not detectable” means that by the state of the art measurement methods used for detecting said label, no signal relating to said label is detectable, and/or said signal is not significant in relation to the background noise generated by said measurement method.

In the above method to stratify patients into Hedgehog dependent patients and Hedgehog independent patients, washing step 4 is to remove unbound and/or unspecifically bound antibodies from step 3. In particular embodiments, said washing step comprises washing with a buffer, e.g. a PBS buffer, and optionally a serum protein, e.g. BSA. Washing step 4 can be repeated as necessary to obtain a suitable signal/noise ratio, e.g. 2 or more, 3 or more, 4 or more times.

In certain embodiments of the above method to stratify patients into Hedgehog dependent cancer patients and Hedgehog independent cancer patients, background signal by unspecific binding of antibodies is excluded by an isotype control. This control can be utilized when working with monoclonal primary antibodies. A comparative sample treated as above is incubated with antibody diluent, supplemented with a non-immune immunoglobulin of the same isotype (for example, IgG₁, IgG2_(A), IgG2_(B), IgM) and concentration as the aforementioned antibody. The sample is then incubated with the labeled antibody and detection reagents. These steps will help ensure that what appears to be specific staining was not caused by non-specific interactions of immunoglobulin molecules with the sample. Examples and a further description of this method can be found in “Tissue Microarrays—Methods in Molecular Biology Volume 664, 2010, pp 113-126, Immunohistochemical Analysis of Tissue Microarrays; Ronald Simon, Martina Mirlacher, and Guido Sauter”.

In the context of the present invention the G protein-coupled receptor Smoothened is interchangeably abbreviated as “Smoothened” and “Smo”.

In the context of the present invention the expression “the activation of the hedgehog signaling pathway” in particular refers to the activation of expression of primary target genes of the Hedgehog signaling pathway, including GLI, HHIP, Ptch, more particularly of GLI expression via the hedgehog pathway. Typically, GLI expression is triggered via binding of hedgehog to the Smo/Ptch (Smoothened/Patched) complex and thereupon GLI expression via signalling by Smo.

In the context of the present invention, the “activation of the hedgehog signaling pathway is independent of signaling by the G protein-coupled receptor Smoothened” as used in the present invention refers to the ability of certain cancers to activate the expression of primary target proteins of the Hedgehog signaling pathway, more particularly of GLI expression independent of signaling via Smo. Instead, in these cases GLI expression is activated by alternate routes, which are independent of the hedgehog signaling pathway as described above.

Known Smoothened inhibitors are for example LDE225 (by Novartis), LEQ506 (by Novartis), Vismodegib (GDC-0449), IPI-926 (by Infinity Pharmaceuticals), BMS-833923 (XL139) (by Bristol Myers Squibb; Exelixis), or PF-04449913 (by Pfizer), N-[3-(1H-Benzimidazol-2-yl)-4-chlorophenyl]-3,4,5-triethoxybenzamide (SANT-2), SANT19, SANT74, aSANT75, (3β,23β)-17,23-Epoxy-3-hydroxyveratraman-11-one (11-ketocyclopamine/Jervine), Cur61414 (by Curis), IPI-269609 (by Infinity), MRT 10, and for example cyclopamine (for further information and further Smoothened inhibitors, see e.g. Peukert S., Miller-Moslin K., ChemMedChem Volume 5, Issue 4, pages 500-512, Apr. 6, 2010).

In the present invention, patients e.g. can be stratified into patients wherein said cancer, or cells of said cancer, do not respond to Smoothened inhibitor therapy, and patients wherein in said cancer, or in the cells of said cancer, respond to Smoothened inhibitor therapy, based on their medical history, i.e. if at a previous point said cancer did not respond or ceased to respond to (wherein said response encompasses the amelioration or stabilization of one or more of the following: Disease state, symptom severity, tumor volume, tumor aggressiveness, propensity to form metastases, tumor malignancy, tumor invasiveness and patient's overall physical state), to Smoothened inhibitor therapy. Alternatively, said stratification can be done by a procedure comprising the steps of

-   1) providing a sample from said patient, wherein said sample     comprises cancer cells from said patient, -   2) preparing two or more cell cultures from said sample, -   3) incubating said cell cultures to increase the number of cells in     order to obtain in each cell culture a cell number which is     sufficient to provide at least 1 μg total RNA, in other embodiments     at least 200 ng or at least 500 ng total RNA, -   4) adding a Smo-inhibitor to all but one of said cell cultures, -   5) adding a Smoothened agonist to said cell cultures, -   6) incubating said cell cultures, -   7) determining the level of GLI expression in said cell cultures, -   8) comparing the level of GLI expression in said cell cultures to     which a Smoothened inhibitor has been added to said sample which no     Smoothened inhibitor has been added, -   9) if the expression of GLI in at least one of said cell cultures to     which a Smoothened inhibitor has been added is lower than the     expression of GLI in said sample which no Smoothened inhibitor has     been added, classifying said patient as patient wherein said cancer,     or the cells of said cancer, respond to Smoothened inhibitor     therapy, and otherwise classifying said patient as patient wherein     said cancer, or cells of said cancer, do not respond to Smoothened     inhibitor therapy.

In the above method, in step 2, cell cultures usually comprise at least a growth medium typically used in the field of cell biology;

in step 3 in particular embodiments, said cell number is at least 500.000, more particularly at least 1.000.000, even more particularly at least 1.500.000 cells, in other particular embodiments, said cell number is at least 100.000, more particularly at least 500.000, even more particularly at least 1.000.000 cells;

in step 4 a Smoothened inhibitor is in particular embodiments added in an amount known to usually at least partially inhibit cellular Smo activity; in the particular embodiments where a Smoothened inhibitor is added to more than one cell culture, a concentration gradient can be prepared by adding different amounts of Smoothened inhibitor to each cell culture;

in step 4 the Smoothened inhibitor is in particular embodiments a small molecule, more particularly a Smoothened inhibitor as described herein,

in step 5 in particular embodiments the Smoothened agonist is added to each cell culture in the same amount with respect to the cell number (i.e. more agonist for a higher cell number) in said cell culture, and in an amount known to usually at least partially activate GLI expression; said amount can for instance be determined in one or more comparative cell cultures comprising cancer cells of the same or similar tissue from a subject which is known to be a as patient wherein said cancer, or the cells of said cancer, respond to Smoothened inhibitor therapy;

in step 6 in particular embodiments said cell cultures are incubated for at least 24 h, 36 h, or 48 h, in this way the cells can express GLI upon the addition of the Smoothened agonist (and in the case that said cancer cells respond to Smoothened inhibitor therapy, allows said cancer cells to degrade GLI);

in steps 7 the level of GLI expression can e.g. be determined with the qPCR and/or Western blot methods described herein;

in step 9, in particular embodiments, to classify said patient as patient wherein said cancer, or the cells of said cancer, respond to Smoothened inhibitor therapy, the difference in the level of GLI expression between said at least one of said cell cultures to which a Smoothened inhibitor has been added and said sample which no Smoothened inhibitor has been added is statistically significantly, e.g. at least 20%, particularly at least 40%, more particularly at least 60%, even more particularly at least 75%, yet even more particularly at least 90%.

Patients can be stratified into patients having cancer wherein in said cancer or in cells of said cancer the activation of the hedgehog signaling pathway is independent of signaling by the G protein-coupled receptor Smoothened and patients having cancer wherein in said cancer or in cells of said cancer the activation of the hedgehog signaling pathway is dependent of signaling by the G protein-coupled receptor Smoothened with common methods. For example, the specific genetic subtype of cancer can be determined and compared with a database. If in said specific genetic subtype of cancer activation of the hedgehog signaling pathway is known to be independent of signaling by the G protein-coupled receptor Smoothened, e.g. in the scientific literature, and in a particular example in the scientific literature cited herein, said patient is classified as Smo independent cancer patient. If in said specific genetic subtype of cancer activation of the hedgehog signaling pathway is known to be dependent of signaling by the G protein-coupled receptor Smoothened, e.g. in the scientific literature, and in a particular example in the scientific literature cited herein, said patient is classified as Smo dependent cancer patient.

Patients can further be stratified into patients having cancer wherein in said cancer or in cells of said cancer the activation of the hedgehog signaling pathway is independent of signaling by the G protein-coupled receptor Smoothened and patients having cancer wherein in said cancer or in cells of said cancer the activation of the hedgehog signaling pathway is dependent of signaling by the G protein-coupled receptor Smoothened by a method comprising the steps of

-   1) providing a sample from said patient, wherein said sample     comprises cancer cells from said patient, -   2) preparing a cell culture from said sample, -   3) incubating said cell culture to increase the number of cells in     order to obtain a cell number which is sufficient to provide at     least 1 μg total RNA, in other embodiments at least 200 ng or at     least 500 ng total RNA, -   4) adding a Smo-inhibitor to said cell culture, -   5) incubating said cell cultures, -   6) determining the level of GLI expression in said cell cultures, -   7) if GLI expression is detectable in said cell culture, classifying     said patient as patient having cancer wherein in said cancer or in     cells of said cancer the activation of the hedgehog signaling     pathway is independent of signaling by the G protein-coupled     receptor Smoothened, and if GLI expression is not detectable in said     cell culture, classifying said patient as patient having cancer     wherein in said cancer or in cells of said cancer the activation of     the hedgehog signaling pathway is dependent of signaling by the G     protein-coupled receptor Smoothened.

In the above method,

in step 2, cell cultures usually comprise at least a growth medium typically used in the field of cell biology;

in step 3 in particular embodiments, said cell number is at least 500.000, more particularly at least 1.000.000, even more particularly at least 1.500.000 cells, in other particular embodiments, said cell number is at least 100.000, more particularly at least 500.000, even more particularly at least 1.000.000 cells;

in step 4 a Smoothened inhibitor is in particular embodiments added in an amount known to usually at least partially inhibit cellular Smo activity; in the particular embodiments where a Smoothened inhibitor is added to more than one cell culture, a concentration gradient can be prepared by adding different amounts of Smoothened inhibitor to each cell culture; in this case, the Smoothened inhibitor can also be and RNA molecule inhibiting the expression of Smo, e.g. siRNA or shRNA, particularly an RNA molecule which specifically and/or selectively binds to the gene encoding for Smo and prevents transcription of said gene (this is typically also known as a Smo knockdown);

in step 4 the Smoothened inhibitor is in particular embodiments a small molecule, more particularly a Smoothened inhibitor as described herein,

in step 5 in particular embodiments in particular embodiments said cell cultures are incubated for at least 12 h, 24 h, or 36 h, in this way the cells can express GLI;

in step 6 the level of GLI expression can e.g. be determined with the qPCR and/or Western blot methods described herein;

in step 7, in particular embodiments, not detectable means that by the state of the art measurement methods used for detecting said label, no signal relating to GLI expression is detectable, and/or said signal is not significant in relation to the background noise generated by said measurement method.

In the context of the present invention “the G protein-coupled receptor Smoothened is not responsive to inhibition by Smoothened inhibitors” means that Smoothened activity is not inhibited by Smoothened inhibitors. Usually, in these cases cancer cells show at least one mutation in a gene encoding for Smoothened, particularly The D473H mutation which results in an amino acid substitution at position 473 in Smoothened, from an aspartic acid (D) to a histidine (H). Said mutation typically leads to an alteration of the Smoothened receptor which prevents binding of Smoothened inhibitors, in particular of known Smoothened inhibitors which target wild type Smoothened. Said mutation may originate from the patient's innate chromosomal setup, i.e. a hereditary trait, or may be acquired at a later point, e.g. by a spontaneous mutation of cancer cell DNA, which is then selected and passed on to further cancer cell generations as a response to therapy with Smoothened inhibitors. Patients can accordingly be classified as patients having cancer wherein in said cancer or in cells of said cancer the activation of the hedgehog signaling pathway is independent of signaling by the G protein-coupled receptor Smoothened by analyzing the genes encoding for Smo in cells from said patient's cancer for the presence of a mutation as described above, which can e.g. be done by the qPCR methods as described herein.

Patients having cancer wherein in said cancer or in cells of said cancer the activation of the hedgehog signaling pathway is dependent of signaling by the G protein-coupled receptor Smoothened can further be stratified into patients having cancer wherein the G protein-coupled receptor Smoothened is not responsive to inhibition by Smo inhibitors and patients having cancer wherein the G protein-coupled receptor Smoothened is not responsive to inhibition by Smo inhibitors by the method described above for stratifying into patients wherein said cancer, or cells of said cancer, do not respond to Smoothened inhibitor therapy, and patients wherein in said cancer, or in the cells of said cancer, respond to Smoothened inhibitor therapy.

In the context of the present invention, said sample from said patient comprising cancer cells from said patient is for instance a biopsy of said patient's cancer.

As used herein, the term “DYRK1B” means an enzyme from the family of serine/threonine kinases, more particularly a member of the Minibrain/DYRK family of kinases which is specified by the UniProt/Swiss-Prot ID Q9Y463; or as Refseq proteins: NP_004705.1, NP_006474.1 and NP_006475.1, and/or an expression product of a gene encoding for DYRK1B as defined herein below. As used herein, the term “DYRK1B” in certain embodiments also includes variants of DYRK1B, such as isoforms, homologs and mutants of DYRK1B, which share at least 95% sequence homology, more particularly at least 97% sequence homology, even more particularly at least 99% sequence homology with DYRK1B as defined above, and in the case of proteins and/or gene expression products in certain embodiments have essentially the same enzymatic activity profile, i.e. process essentially the same substrates as DYRK1B as defined above, wherein however the enzymatic activity of said variants of DYRK1B may differ (i.e. be higher or lower than) from DYRK1B as defined above by up to two orders of magnitude, particularly up to one order of magnitude, more particularly up to a factor of 2. A particular variant of DYRK1B which is comprised by the present invention is Mirk, which typically is located in skeletal muscle tissue, whereas DYRK1B typically is located in brain tissue.

In the context of the present invention said at least one protein playing a role in the hedgehog signaling pathway can e.g. be selected from the group comprising Patched, GLI, Smoothened, HHIP, Hedgehog and SUFU.

In the context of the present invention, the term “GLI” refers to members of the GLI protein family, such as GLI1, GLI2, GLI3 in particular embodiments and, unless specified otherwise particularly to GLI1.

In general, and unless specified otherwise, the proteins, genes and/or gene expression products as defined herein in certain embodiments also include variants of said proteins, genes and gene expression products, such as isoforms, homologs and mutants thereof, which share at least 95% sequence homology, more particularly at least 97% sequence homology, even more particularly at least 99% sequence homology with, the proteins, genes and/or gene expression products as defined herein, and in the case of proteins and/or gene expression products in certain embodiments have essentially the same enzymatic activity as, the proteins and/or gene expression products as defined herein, wherein however the enzymatic activity of said variants may differ (i.e. be higher or lower than) from the proteins, and/or gene expression products as defined herein by up to two orders of magnitude, particularly up to one order of magnitude, more particularly up to a factor of 2.

As used herein, the term “DYRK1B inhibitor” or “compound which inhibits the activity of DYRK1B” means a compound which is capable of inhibiting the enzymatic activity of DYRK1B in vitro and/or in vivo, e.g. in a patient in need thereof, particularly with an inhibitory concentration IC₅₀ of 50 mM or lower, more particularly with an inhibitory concentration IC₅₀ of 20 mM or lower, even more particularly with an inhibitory concentration IC₅₀ of 5 mM or lower, yet even more particularly with an inhibitory concentration IC₅₀ of 1 mM or lower, e.g. in the kinase assay described herein. The chemical nature of the DYRK1B inhibitor is not particularly limited and can for example be selected from the group comprising synthetic compounds, naturally occurring compounds, peptides, proteins, antibodies, and small molecules. For the avoidance of doubt, it is mentioned that the above IC₅₀ values are erroneous, which a skilled person readily recognizes, because an inhibitory concentration IC₅₀ of 50 mM, 20 mM, 5 mM, 1 mM or lower is apparently not very potent for treatment of diseases. Thus the above values should, at least in a specific embodiment be read as IC₅₀ of 50 μM or lower, more particularly with an inhibitory concentration IC₅₀ of 20 μM or lower, even more particularly with an inhibitory concentration IC₅₀ of 5 μM or lower, yet even more particularly with an inhibitory concentration IC₅₀ of 1 μM or lower.

In certain embodiments of the present invention, said DYRK1B inhibitor is selective and/or specific for DYRK1B. In certain embodiments of the present invention, said DYRK1B inhibitor is non-selective and/or non-specific for DYRK1B. It is apparent that non-selectivity and/or non-specificity is acceptable, as long as no intolerable side-effect occurs due to the non-selectivity and/or non-specificity of the DYRK1B inhibitor. In this context, “intolerable side-effect” means an effect of the addition or administration of the DYRK1B inhibitor which is different from the inhibition of DYRK1B, e.g. the inhibition of one or more further enzymes, and which conflicts with the objective to be achieved by the addition or administration of the DYRK1B inhibitor to such a degree that no acceptable results may be achieved. Examples are for instance a pharmaceutical side effect which would prevent the use of the DYRK1B inhibitor for therapeutic purposes, such as high toxicity, cancerogenity or the like. As one example of tolerable non-selectivity and/or non-specificity, the DYRK1B inhibitor may inhibit DYRK1A in addition to DYRK1B. DYRK1A function is largely dispensable in GLI-driven cancer cells. Off-target effects of DYRK1B inhibitors will not be critical as inhibition of DYRK1A—if at all—will enhance the GLI-antagonizing effect of DYRK1B inhibition in cancer cells. This effect is for instance credible in view of the results shown in Mao et al., J. Biol. Chem. 2002, 277, 38, 35156-35161, in particular FIGS. 4 and 6.

It is also within the general scope of the present invention to inhibit DYRK1B activity by inhibiting the expression of a gene encoding for DYRK1B. This can be achieved by inhibiting expression of said gene on DNA level and/or RNA level, thus lowering the amount of DYRK1B produced by said gene and consequently lowering the overall activity of DYRK1B in a subject in which the expression of said gene is inhibited. The methods by which the expression of said gene is inhibited are not particularly limited, and may comprise the addition or administration of an agent selected from the group comprising siRNA, shRNA, miRNA, antisense-oligonucleotides.

As used herein, the expression that in a cancer or cells in a cancer the “hedgehog signaling pathway is independent of signaling by the G protein-coupled receptor Smoothened” means that the proliferation of a certain cancer entity is independent of the G protein-coupled receptor Smoothened and thus not affected, or at least not substantially affected, by inhibition of the G protein-coupled receptor Smoothened. Accordingly, patients suffering from such cancer cannot be treated and do not benefit by therapeutic approaches which are directed to inhibition of the G protein-coupled receptor Smoothened.

As used herein, the term “hedgehog signaling pathway” means a cellular signaling pathway comprising an interaction with a protein of the family known as hedgehog proteins, such as e.g. the proteins commonly known as “sonic hedgehog” (UniProtKB/Swiss-Prot: Q15465), “indian hedgehog” (UniProtKB/Swiss-Prot: Q14623) and “desert hedgehog” (UniProtKB/Swiss-Prot: 043323) (Ingham and McMahon, 2001).

As used herein, the term “treating” or “treatment” encompasses the amelioration or stabilization of one or more of the following: Disease state, symptom severity, tumor volume, tumor aggressiveness, propensity to form metastases, tumor malignancy, tumor invasiveness and patient's overall physical state.

As used herein, the term “therapeutically effective amount” means an amount, e.g. of a compound, which upon administration to a patient in need thereof results in a therapeutic effect on the disease to be treated. Such therapeutic effects, e.g. in cancer therapy, may comprise an effect on diseased tissue or cells including changes in tumor size, metabolic activity, cell viability, blood supply of the tumor, i.e. angiogenesis, composition of the tumor, e.g. relationship of cells comprising the tumor e.g. tumor cells, immune cells, fibroblasts and endothelial cells; and an effect on the patient's medical state including improvements in clinical status, health status, progression or stabilization of disease, increased time of progression free survival, cure of disease, enhanced overall survival, delay of disease progression and alleviation of symptoms. Such effects usually do not occur immediately after administration of a compound and may be delayed, e.g. by hours, days, weeks or months, depending e.g. on the specific patient, type of disease and overall situation under which the therapy is administered.

As used herein, the term “sample” in principle comprises samples from natural sources, such as a sample obtainable from a mammal, and artificial samples, which are obtainable by admixing several ingredients, wherein said ingredients may or may not be derived from natural sources, and may e.g. comprise ingredients selected from the group comprising synthetic and/or natural proteins, peptides, oligo- or polynucleic acids, etc. In certain embodiments, samples are from natural sources, which include bodily fluids and/or tissue samples, such as bodily fluid and/or a tissue sample obtainable from mammals. Said samples from natural sources can be used in the present invention with or without further processing after being obtained from their source, e.g. a mammal. Such processing can for instance comprise separation, fractionation, dilution, dispersion, mechanical treatment such as sonification, or grinding, concentration, removal of certain components of said sample, or addition of compounds, such as salts, buffers, detergents, etc.

As used herein, the term “bodily fluid” or “body fluid” specifies a fluid or part of a fluid originating from the body of a patient, including fluids that are excreted or secreted from the body of the patient, including but not limited to blood, including peripheral blood, serum, plasma, urine, interstitial fluid, liquor, aqueous humour and vitreous humour, bile, breast milk, cerebrospinal fluid, endolymph, perilymph, ejaculate, gastric juice, mucus, peritoneal fluid, pleural fluid, saliva, sweat, tears and vaginal secretion, particularly peripheral blood, serum, plasma and urine. Said bodily fluid itself may or may not comprise diseased and/or non-diseased cells.

As used herein, the term “tissue sample” specifies a non-fluid material or solid originating from the body of a patient. Tissue samples include, but are not limited to samples of bone material, bone marrow, skin, hair follicle, mucosa, brain, cartilage, muscles, lung, kidney, stomach, intestines, bladder and liver. Said tissue sample itself may or may not comprise diseased cells, and may for instance be a sample taken from a diseased region of a patient's body, such as a biopsy of a tumor. In certain embodiments the tissue sample is selected from skin, hair follicle or oral mucosa.

In the embodiments of the present invention, the sample is obtained from the patient by any method and/or means commonly known to the skilled person in the field of medicine, e.g. in certain embodiments blood sample taking by venipuncture.

As used herein, the term “peripheral blood” specifies blood obtained from the circulation remote from the heart, i.e. the blood in the systemic circulation, as for example blood from acral areas.

As used herein, the term “whole blood” specifies unmodified blood comprising cells and fluid, as obtained from the donor of said blood, such as a patient.

As used herein, the term “patient” specifies a subject which is suspected of having a disease or disorder, in certain embodiments having a medical condition, which may require treatment. In certain embodiments of the present invention, the patient is a cancer patient, i.e. a subject suffering from cancer. The patient may have received prior treatment for the disease in question, e.g. in the case of a cancer patient by radiation or chemotherapy, or the patient's disease may be untreated prior to the application of the embodiments of the present invention to said patient.

As used herein, the term “small molecule” is to be understood as commonly used in the field of pharmacology and means a low molecular weight organic compound which is not a polymer, and which usually has a molecular weight of about 800 Daltons or lower, particularly, 700 Daltons or lower, more particularly 600 Daltons or lower, even more particularly 500 Daltons or lower. From a functional point of view, a small molecule has to molecular weight which allows the molecule to rapidly diffuse across cell membranes and/or reach the interior of a mammalian cell.

As used herein, the term “gene encoding for DYRK1B” means a gene identified by the NCBI reference sequence (Refseq mRNAs NM_004714.1, NM_006483.1, NM_006484.1, or the Ensemble transcripts ENST00000323039(uc002omj.2) ENST00000348817(uc002omi.2) ENST00000430012(uc002omk.2 uc002oml.2))

As used herein, the term “inhibited on the DNA level and/or on the RNA level” means that the intracellular level of the protein of interest, in the case of the present invention DYRK1B, is diminished by inhibiting the expression of the gene encoding for the protein of interest. This can be achieved either by an inhibition on the DNA level, i.e. by inhibiting transcription of the gene encoding for the protein of interest, or by an inhibition on the RNA level, i.e. by inhibiting translation of an RNA transcribed from the gene encoding for the protein of interest. The methods by which inhibition on the DNA level and/or on the RNA level can be achieved are well known to the skilled person and any such well known method which is suitable for the purposes of the present invention, e.g. for therapeutic use in a patient suffering from cancer, or for in vitro use such as in an assay, can be applied in the embodiments of the present invention.

In the context of the present invention, a mammal is in certain embodiments a human.

As used herein, the terms “inhibit DYRK1B”, “inhibition of DYRK1B” and “DYRK1B inhibition” are used interchangeably and mean that the enzymatic activity of DYRK1B is diminished, which results in a diminished turnover rate with respect to the conversion of DYRK1B substrates by DYRK1B, which can particularly be determined by measuring a reduction in the level of GLI expression by the methods described herein, e.g. the qPCR or Western Blot methods described herein. Said reduction in the level of GLI expression is in particular embodiments at least 50%, more particularly at least 70%, even more particularly at least 80%, yet even more particularly at least 90%.

As used herein, the term “formation of resistance of cancer cells against chemotherapeutic agents” means that over the course of a treatment with a chemotherapeutic agent, the treated cancer cells develop a resistance against said chemotherapeutic agent, i.e. become non-responsive to said chemotherapeutic agent. The result of resistance against said chemotherapeutic agent is that the cancer cells will proliferate, irrespective of continued therapy with said chemotherapeutic agent. Usually, such resistance against a chemotherapeutic agent is not reversible, and usually, in such cases of resistance against a chemotherapeutic agent, therapy has to be changed to a different treatment regimen, e.g. encompassing the administration of a different chemotherapeutic agent, radiotherapy, or the like.

In the embodiments of the present invention, in particular wherein a DYRK1B inhibitor is administered in an amount which is effective to inhibit DYRK1B said DYRK1B inhibitor is administered in an amount which is effective to inhibit DYRK1B by for example by at least 50%, more particularly at least 75%, even more particularly at least 90%, inhibition of DYRK1B can be determined in vitro by a DYRK1B kinase assay and ex vivo by measuring the expression level of a primary target gene of the hedgehog signaling pathway, e.g. GLI, particularly in a sample obtained from a patient; this can be done by the methods described herein.

In further particular embodiments of the present invention, the hedgehog signaling pathway is inhibited, which can be determined by measuring the expression level of a primary target gene of the hedgehog signaling pathway, e.g. GLI, particularly in a sample obtained from a patient; this can be done by the methods described herein, e.g. qPCR or Western Blot.

In further particular embodiments of the present invention, the cellular expression of GLI, particularly GLI1 is inhibited.

In further particular embodiments of the present invention, the hedgehog signaling pathway-mediated cellular expression of GLI, particularly GLI1 is inhibited.

Furthermore, particular genetic subtypes of cancer of certain embodiments of the present invention are listed in the following; reference documents with further information are indicated in parentheses; for said genetic subtypes of cancer, among others, it is known that activation of the hedgehog signaling pathway is independent of signaling by the G protein-coupled receptor Smoothened:

esophagus/GI (Wang, Y., et al. (2012). Canc. cell 21(3) 374-387. doi:10.1016/j.ccr.2011.12.028);

gastrointestinal (Berman, D. M., et al. (2003). Nature, 425(6960), 846-851. doi:10.1038/nature01972);

gastrointestinal stromal tumors (Pelczar, P. et al. (2013). Gastroenterology, 144(1), 134-144.e6. doi:10.1053/j.gastro.2012.09.061);

pancreas (Nolan-Stevaux, O. et al. (2009). Genes & Development, 23(1), 24-36. doi:10.1101/gad.1753809; Feldmann, G., et al. (2007). Cancer Research, 67(5), 2187-2196. doi:10.1158/0008-5472.CAN-06-3281; Karhu, R. et al. (2006) Genes, chromosomes & cancer 45, 721-730; Merchant, A. A., and Matsui, W. (2010) Clin. Canc. Res. 16, 3130-3140);

prostate (Karhadkar, S S et al. (2004). Nature, 431(7009), 707-712. doi:10.1038/nature02962; Sanchez, P., et al. (2004). PNAS, 101(34), 12561-12566. doi:10.1073/pnas.0404956101);

biliary tract (Berman, D M et al. (2003). Nature, 425(6960), 846-851. doi:10.1038/nature01972);

bladder/urogenital (Fei, D L et al. Cancer Res. 2012 Sep. 1; 72(17):4449-58);

basal cell carcinoma/skin (Hahn, H., et al. (1996). Cell, 85(6), 841-851);

medulloblastoma/brain (Goodrich, L. V., et al. (1997). Science, 277(5329), 1109-1113);

rhabdomyosarcoma (Ecke, I. et al. (2008). Molecular carcinogenesis, 47(5), 361-372. doi:10.1002/mc.20394; Deng, X. et al. (2006) Cancer research 66, 4149-4158; Friedman, E. (2011) Sarcoma 2011, 260757, doi:10.1155/2011/260757; Jin, K. et al. (2007) Cancer research 67, 7247-7255; Mercer, S. E. et al. (2006) Cancer research 66, 5143-5150; Yang et al., Carcinogenesis. 2010 April; 31(4):552-558);

glioma/brain (Clement, V., et al. (2007). Current biology: CB, 17(2), 165-172. doi:10.1016/j.cub.2006.11.033);

small-cell lung cancer/lung (Watkins, D. N. et al. (2003). Nature, 422(6929), 313-317. doi:10.1038/nature01493; Park K S, et al. Nat Med. 2011 Oct. 9; 17(11):1504-8. doi: 10.1038/nm.2473.);

oral squamous cell carcinoma (Yan M, et al. Oral Oncol. 2011 June; 47(6):504-9. doi: 10.1016/j.oraloncology.2011.03.027. Epub 2011 May 4);

melanoma/skin (Stecca B et al. (2007) PNAS 104(14) 5895-5900 doi:10.1073/pnas.0700776104);

colorectal cancer/GI (Varnat, F., et al. (2009). EMBO molecular medicine, 1(6-7), 338-351. doi:10.1002/emmm.200900039; Deng, X. et al. (2006) Cancer research 66, 4149-4158; Friedman, E. (2011) Sarcoma 2011, 260757, doi:10.1155/2011/260757; Jin, K. et al. (2007) Cancer research 67, 7247-7255; Mercer, S. E. et al. (2006) Cancer research 66, 5143-5150; Yang et al., Carcinogenesis. 2010 April; 31(4):552-558);

non-small cell lung cancer/lung (Yuan, Z., et al. (2007). Oncogene, 26(7), 1046-1055. doi:10.1038/sj.onc.1209860);

osteosarcoma/bone (Bovée, J. V. M. G., et al. (2010). Nature Reviews Cancer, 10(7), 481-488. doi:10.1038/nrc2869; Ho, L., et al. (2009). Cancer cell, 16(2), 126-136. doi:10.1016/j.ccr.2009.05.013; Friedman, E. (2011) Sarcoma 2011, 260757, doi:10.1155/2011/260757; Jin, K. et al. (2007) Cancer research 67, 7247-7255; Mercer, S. E. et al. (2006) Cancer research 66, 5143-5150; Yang et al., Carcinogenesis. 2010 April; 31(4):552-558);

glioblastoma/brain (Clement, V., et al. (2007). Current biology: CB, 17(2), 165-172. doi:10.1016/j.cub.2006.11.033);

chronic lymphacytic leukemia/blood (Desch, P., et al. (2010). Oncogene, 1-11. doi:10.1038/onc.2010.243);

chronic myeloid leukemia/blood (Dierks, C., et al. (2008). Cancer cell, 14(3), 238-249. doi:10.1016/j.ccr.2008.08.003);

multiple myeloma/blood (Peacock C D, et al. PNAS 2007 Mar. 6; 104(10):4048-53);

ovarian cancer/urogenital (McCann C K, et al. PLoS One. 2011; 6(11):e28077. doi: 10.1371/journal.pone.0028077. Epub 2011 Nov. 29; Friedman, E. (2007) Journal of cellular biochemistry 102, 274-279, 2007; Karhu et al., Genes Chromosomes Cancer. 2006 August; 45(8):721-730);

meningioma/brain (Clark, V. E. et al., Science, 2013 Jan. 24, Epub ahead of print, PMID (PubMed-ID) 23348505—as supplied by publisher; Aavikko M. et al., Am J Hum Genet, 2012 Sep. 7, 91(3), 520-526);

liver/GI (Arzumanyan A. et al. Cancer Res. 2012 Nov. 15; 72(22):5912-20);

liver (Huang, S., et al. (2006). Carcinogenesis, 27(7), 1334-1340. doi:10.1093/carcin/bgi378).

It is apparent that the embodiments of the present invention as described herein can be combined to form further particular embodiments of the present invention.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1: Western blot analysis (upper panel) and qPCR analysis (lower panel) of medulloblastoma cells (DAOY) treated with solvent (DMSO) or SAG (100 nM, 48 hours under starving conditions) transduced with shRNA targeting DYRK1A (shDYRK1A) or DYRK1B (shDYRK1B). DYRK1B targeting efficiently reduces GLI1 and GLI2 protein expression as well as GLI1 and PTCH1 mRNA expression. Non-target shRNA (shContr) served as control. Beta Actin served as loading control for western blot analysis. qPCR data represent fold mRNA changes compared to solvent (DMSO) treated control.

FIG. 2: Western blot analysis (upper panel) and qPCR analysis (lower panel) of DAOY cells stimulated for 48 hours with 100 nM SAG under starving conditions. Concomitant treatment with Harmine significantly inhibited GLI1 protein and PTCH1 mRNA expression. The SMO inhibitor GDC-0449 (Vismodegib) served as positive control, Beta Actin as loading control for western blot analysis. qPCR data represent fold mRNA change values compared to solvent (DMSO) treated controls. The final DMSO concentration in all wild-type DAOY samples was 0.2%, in all other cell line experiments including DAOY shSUFU cells 0.1%.

FIG. 3: Western blot analysis of DAOY cells treated with SAG as described in FIGS. 1 and 2. Concomitant treatment with DYKR1 inhibitor Compound P (Cpd P) significantly inhibited GLI1 protein expression.

FIG. 4: Western blot analysis of RNAi knockdown (left panel) and pharmacological inhibition (right panel) of DYRK1b in mouse Ptch−/− BCC cells (BSZ2) showing reduced GLI1 expression upon RNAi mediated knockdown and pharmacological inhibition of DYRK1b by Harmine (the left panel demonstrates efficient DYRK1B knockdown and correlation of GLI repression). Beta Actin served as loading control for western blot analysis. *p<0.05, **p<0.01.

FIG. 5: RNAi mediated knockdown of SUFU (shSUFU) in DAOY cells leads to SMO independent activation of GLI1 expression that is resistant to SMO inhibition. Western blot (left panel) and qPCR analysis (right panel) showing SMO-independent HH target gene activation i.e. GLI1 protein and PTCH1 mRNA expression. In SUFU knockdown cells, activation of GLI1 and PTCH1 expression is resistant to treatment with GDC-0449. The cells nonetheless remain sensitive to treatment with GANT61 (shown only for PTCH1 expression). qPCR data represent fold change values compared to non-target shRNA (shContr).

FIG. 6: Western blot analysis showing effects of knockdown of DYRK1A/B alone or in combination with shSUFU on GLI1 and GLI2 protein expression in DAOY cells. Only DYRK1B inhibition reduces GLI1 protein levels in SUFU depleted cells.

FIG. 7: Left panel: Western blot analysis of PANC1 cells showing resistance of GLI1 protein expression to SMO inhibition by GDC-0449 treatment. Middle panel: Effect of RNAi mediated knockdown of DYRK1A or DYRK1B on GLI1, GLI2, Beta Catenin, STAT3 and STATS. Right panel: Effect of Harmine treatment on GLI1, Beta Catenin and STAT3 expression. In both cases, only DYRK1B inhibition reduces GLI1 and/or GLI2 protein levels in PANC1 cells. The general transcription factors Beta Catenin, STAT3 and STATS are essentially unaffected by DYRK1A and DYRK1B inhibition.

FIG. 8: Left panel: EWS (Ewing sarcoma) cell line (A673) Western blot analysis showing the effect of RNAi mediated knockdown of DYRK1A or DYRK1B on GLI1 and GLI2. Right panel: Effect of Harmine and GDC-0449 on GLI1, Beta Catenin and STAT3 protein expression. In both cases DYRK1B inhibition reduces GLI1 and/or GLI2 protein levels in EWS cells. The general transcription factor Beta Catenin is essentially unaffected by DYRK1A and DYRK1B inhibition.

FIG. 9: Effect of RNAi mediated knockdown of DYRK1A or DYRK1B on clonal growth of tumor-initiating PANC1 pancreatic cancer cells in anchorage-independent 3D cultures. The right panel represents the quantification of the 3D cultures shown in the left panel. Data represent the mean values of at least 3 independent experiments (error bars represent SEM. *** p<0.001). Only DYRK1B repression reduces colony numbers.

FIG. 10: Results of pharmacological inhibition of DYRK1 on clonal growth of tumor-initiating PANC1 pancreatic cancer cells in anchorage-independent 3D cultures. The right panel represents the quantification of the 3D cultures shown in the left panel. Data represent the mean values of at least 3 independent experiments (error bars represent SEM. *** p<0.001). Only DYRK1 inhibition by Harmine but not SMO inhibition by GDC-0449 reduces sphere numbers.

FIG. 11: Results of pharmacological inhibition of DYRK1 on clonal growth of tumor-initiating L3.6p1 pancreatic cancer cells in anchorage-independent 3D cultures. The right panel represents the quantification of the 3D cultures shown in the left panel. Data represent the mean values of at least 3 independent experiments (error bars represent SEM. *** p<0.001). Only DYRK1 inhibition by Harmine but not SMO inhibition by GDC-0449 reduces sphere numbers.

FIG. 12: Effect of RNAi mediated knockdown of DYRK1B on in vivo tumor growth of pancreatic cancer cells. 1×10⁶ pancreatic cancer cells (PANC1) either lentivirally transduced with non-target control shRNA (shContr) or with shRNA specific for DYRK1B (shDYRK1B) were grafted onto nude mice and tumor growth was monitored over a period of 12 weeks. (n=7; Error bars represent SEM. *p<0.05, **p<0.01).

FIG. 13: Effect of RNAi mediated knockdown of DYRK1B on in vivo tumor growth of pancreatic cancer cells. 1×10⁵ pancreatic cancer cells (L3.6p1) either lentivirally transduced with non-target control shRNA (shContr) or with shRNA specific for DYRK1B (shDYRK1B) were grafted onto nude mice and tumor growth was monitored over a period of 3 weeks. (n=8; Error bars represent SEM. *p<0.05, **p<0.01).

EXAMPLES Material and Methods

In all cases, kits and specific reagents were used according to the instructions in the respective manuals provided by the manufacturer, unless stated otherwise. The methods described in said manuals are incorporated herein by reference.

Cell Culture and Inhibitor Treatments

DAOY cells (ATCC) were grown in MEM media (PAA Laboratories, Germany) supplemented with 10% fetal bovine serum (FBS) (PAA Laboratories, Germany), penicillin (62.5 μg/ml) and streptomycin (100 mg/ml) at 37° C. in an atmosphere containing 5% CO₂. BSZ2 cells (So et al., 2006) were grown in 154-CF media (Life Technologies, UK) supplemented with 2% heat inactivated (56° C. for 30 minutes) and chelexed fetal bovine serum (FBS) (PAA Laboratories, Germany) (FBS was chelexed by adding to 50 mL FBS 10 g Chelex 100 (Bio-Rad Laboratories GmbH, Germany) followed by stirring for 60 min at RT; chelexed FBS was sterile filtered prior to use), penicillin (62.5 μg/ml) and streptomycin (100 mg/ml) at 37° C. in an atmosphere containing 5% CO₂. Panc-1 and A673 cells (ATCC) were grown in DMEM media (PAA Laboratories, Germany) supplemented with 10% fetal bovine serum (FBS) (PAA Laboratories, Germany), penicillin (62.5 μg/ml) and streptomycin (100 mg/ml) at 37° C. in an atmosphere containing 5% CO₂. For 3-dimensional (3D) cultures, 5×10³ cells were seeded in 12-well plates in 0.4% select agar on top of 0.5% bottom select agar (Life Technologies, Germany) according to standard protocols of anchorage independent growth assays (Eberl et al., 2012). 3D cultures were grown for 4-6 weeks at 37° C. in a humidified atmosphere containing 5% CO₂. Colony formation was documented on a stereomicroscope with Cell̂D Image capture system (Olympus) and quantified using Colony Counter Software (Microtec Nition, Japan). SAG (smoothened agonist, N-Methyl-N′-(3-pyridinylbenzyl)-N′-(3-chlorobenzo[b]thiophene-2-carbonyl)-1,4-diaminocyclohexane) (Axxora LCC), Harmine (Fisher Scientific), GDC-0449 (LC Laboratories), GANT61 (Merck Chemicals Ltd.) and 4SC Compound P (4SC Discovery GmbH, Germany) were dissolved in DMSO.

The final DMSO concentration in all wild-type DAOY samples was 0.2%, in all other cell line experiments including DAOY shSUFU cells 0.1%.

The chemical structure and chemical name of 4SC compound P are shown below:

RNA Interference and Lentiviral Transduction

In the examples of the present invention, RNAi knockdown experiments were facilitated by lentiviral RNAi production and transductions, which were carried out as described in Kasper et al, 2007, which is incorporated herein by reference (Kasper et al., 2007). The following shRNA constructs (Sigma Aldrich mission TRC library) were used and the following abbreviations are used herein: shRNA DYRK1A (TRCN0000000526), shRNA DYRK1B-1 (TRCN0000002139), shRNA DYRK1B-2 (TRCN0000355722), shSUFU (TRCN0000019466) and control scrambled shRNA (SHC002). The functionality of the shRNAs was validated by Western blot analysis. Transduced cells were selected for puromycin resistance prior to further analysis.

RNA Isolation, qPCR and Western Blot Analysis

Total RNA was isolated using TRI-reagent (Molecular Research Center Inc.) followed by LiCl purification step. For this purpose, total RNA was dissolved in 100 mM Hepes pH 7.4/6M Urea and selectively precipitated by adding LiCl to a final concentration of 3M and incubating for 2 h at −20° C. Precipitated and purified RNA was used for cDNA synthesis using Superscript II reverse transcriptase (Life Technologies, Germany) according to the manufacturer's instructions. qPCR was done on a Rotorgene3000 (Qiagen, Germany) using iQ Sybr Green Supermix reagent (Bio-Rad Laboratories, UK). For qPCR analysis the following primer pairs (Microsynth AG, Switzerland) were used:

human GLI1: (SEQ ID NO: 1) forward 5′ TCTGGACATACCCCACCTCCCTCTG 3′ (SEQ ID NO: 2) reverse 5′ ACTGCAGCTCCCCCAATTTTTCTGG 3′ human PTCH1: (SEQ ID NO: 3) forward 5′ TCCTCGTGTGCGCTGTCTTCCTTC 3′ (SEQ ID NO: 4) reverse 5′ CGTCAGAAAGGCCAAAGCAACGTGA 3′

For Western blot analysis, cells were lysed in Laemmli buffer supplemented with PhosStop (Roche, Germany) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, Germany). Proteins were visualized with horseradish peroxidase-conjugated secondary antibodies in combination with enhanced chemiluminescence detection system (GE Health Care, USA).

The following antibodies were used:

Primary antibodies: Human and mouse GLI1: Cell Signaling Technology (Boston, Mass., USA), GLI1 (V812) #2534, polyclonal rabbit, 1:1000; Human GLI2: Santa Cruz Biotechnology, (Santa Cruz, Calif., USA), GLI2 (H300) sc-28674, polyclonal rabbit, 1:1000; Human and mouse DYRK1A: Cell Signaling Technology (Boston, Mass., USA), DYRK1A #2771, polyclonal rabbit, 1:1000; Human and mouse DYRK1B: Cell Signaling Technology (Boston, Mass., USA), DYRK1B #2703, polyclonal rabbit, 1:1000; Human SUFU: Santa Cruz Biotechnology, (Santa Cruz, Calif., USA), SUFU (C-15) sc-10933, polyclonal goat, 1:200; Human STAT3: BD Transduction Laboratories, (BD Biosciences, NJ, USA), STAT3 610189, monoclonal mouse, 1:5000; Human STATS: Cell Signaling Technology (Boston, Mass., USA), STATS (3H7) #9358, monoclonal rabbit, 1:1000; Human Beta Catenin: Cell Signaling Technology (Boston, Mass., USA), β-Catenin #9587, polyclonal rabbit, 1:1000; Human and mouse Beta Actin: Santa Cruz Biotechnology (Santa Cruz, Calif., USA), β-Actin sc-47778, monoclonal mouse, 1:1000.

Secondary antibodies: Anti-mouse IgG, horse, HRP-linked: Cell Signaling Technology (Boston, Mass., USA) #7076, 1:3000; Anti-rabbit IgG, goat, HRP-linked: Cell Signaling Technology (Boston, Mass., USA) #7074, 1:3000; Anti-goat IgG, chicken, HRP-linked: Santa Cruz Biotechnology (Santa Cruz, Calif., USA) sc-2953, 1:5000.

Xenografts: For in vivo tumor growth studies 1×10⁶ PANC1 or 1×10⁶ L3.6p1 (Bruns et al., 1999) pancreatic cancer cells in 25% Matrigel (BD Biosciences, NJ, USA) were injected subcutaneously into the lower flanks of Foxn 1 nu/nu nude mice (Charles River Laboratories, USA). Tumor volume was measured with a caliper and calculated according to the formula [4/3×π×(length/2)×(width/2)×(height/2).

Example 1—Genetic and Pharmacological Inhibition of DYRK1B in Human Cancer Cells Example 1A

To address the effect of selected gene perturbations on canonical endogenous Hedgehog signaling in a human cancer cell line, the human medulloblastoma cell line (DAOY, ATCC ident. HTB-186) showing full responsiveness to Hedgehog activation by Smoothened agonist (SAG) as evidenced by activation of the direct Hedgehog target gene GLI1, a reliable and robust indicator of Hedgehog signaling pathway activity (FIG. 1) (Chen et al., 2002).

As shown in FIG. 1, RNAi knockdown of DYRK1A only moderately interfered with SAG induced (100 nM for 48 h under starving conditions) activation of GLI1 and PTCH1 expression. Notably, DYRK1B RNAi knockdown with shRNA DYRK1B-1 led to increased inhibition of SAG-induced pathway activation and HH target gene expression, respectively. This result was confirmed using shRNA for DYRK1B-2. Furthermore, knockdown of DYRK1B significantly decreased GLI2 protein levels independent of SAG treatment (FIG. 1 left panel). In the HH-responsive human medulloblastoma cell line DAOY, DYRK1 function is required for canonical HH signaling, with DYRK1B being the essential DYRK kinase.

Example 1B

SAG stimulated DAOY cells were treated with Harmine (7-MeO-1-Me-9H-pyrido[3,4-b]-indole) (dissolved at room temperature as 25 mM stock solution in DMSO and used at final concentration of 10 μM or 25 μM), a naturally occurring DYRK1 inhibitor, as well as with Compound P (Cpd P, dissolved at room temperature in DMSO as 20 mM stock solution and used at final concentration of 1 μM or 5 μM, respectively), a small molecule inhibitor of DYRK1, which is structurally unrelated to Harmine. Both Harmine and Cpd P prevented HH signaling as evidenced by a significant reduction of GLI target gene activation (FIGS. 2 and 3 respectively). For comparison, we also analyzed the effect of GDC-0449 (Vismodegib) treatment on SAG-mediated Hedgehog signaling pathway activation and target gene expression. As expected, the Smoothened antagonist GDC-0449 (dissolved at room temperature in DMSO as 10 mM stock solution and used at a final concentration of 0.504) efficiently blocked SAG-induced Hedgehog signaling pathway activation as evidenced by inhibition of GLI1 protein expression.

Example 1C

The above experiments were repeated with murine Ptch/basal cell carcinoma (BCC) cells (BSZ2) (So et al., 2006) showing constitutive, Smoothened-dependent canonical activation of Hh/GLI signaling. FIG. 4 shows that like in human HH-responsive DAOY cells, RNAi mediated genetic and pharmacological perturbation of DYRK1B function led to a decrease in GLI1 expression in BSZ2 cells. These findings confirm the critical role of DYRK1B in promoting canonical Hh/GLI signaling independent of the cellular model of Hh signaling.

Example 2—Targeting DYRK1B Inhibits Smoothened-Independent GLI Activity in Human Cancer Cells

Resistance of DAOY cells to SMO-inhibitors by RNAi mediated inhibition of SUFU expression, a critical negative regulator of GLI proteins acting downstream of SMO was generated. As shown in FIG. 5, stable lentiviral RNAi-mediated SUFU knockdown (for details see Material and Methods, RNA interference and lentiviral transduction) in DAOY cells resulted in activation of GLI1 expression. Notably and unlike control knockdown cells, GLI1 expression in SUFU depleted DAOY cells was unaffected by GDC-0449 (2-chloro-N-(4-chloro-3-(pyridin-2-yl)phenyl)-4-(methylsulfonyl)benzamide; INN Vismodegib) treatment, indicating resistance to SMO-inhibition. By contrast, SUFU-knockdown cells retained their sensitivity to the GLI antagonist GANT61 (2,2′-[[Dihydro-2-(4-pyridinyl)-1,3 (2H,4H)-pyrimidinediyl]bis-(methylene)]bis[N,N-dimethylbenzenamine), as evidenced by down-regulation of GLI1 and PTCH1 mRNA expression.

Of note, RNAi-mediated inhibition of DYRK1B prevented GLI1 expression even in SUFU-depleted, SMO-inhibitor resistant DAOY cells, while DYRK1A inhibition did not reduce GLI1 activation in this cellular model (FIG. 6).

Example 2 A

The function of DYRK1 isoforms in SMO-independent/GLI-dependent pancreatic cancer and Ewing's sarcoma (EWS) cell lines PANC1 and A673, respectively, was analyzed. PANC1 cells display SMO-independent, TGFβ-driven expression of GLI1 and GLI2, whereas A673 cells express GLI1 as a direct transcriptional target of the fusion oncoprotein EWS/FLI1s. The inability of GDC-0449 treatment (0.5 μM) to decrease GLI expression (FIG. 7 left panel, FIG. 8 right panel) demonstrates SMO-independent expression of GLI in both cell lines. Importantly and in line with the presented data on SUFU-depleted medulloblastoma cells, genetic perturbation of DYRK1B, but not of DYRK1A, led to a reduction of endogenous GLI1 and GLI2 protein expression. Inhibition of GLI1/2 expression by DYRK1B targeting is a specific effect, as DYRK1B knockdown did not alter the abundance of other transcriptional regulators such as STAT3, STATS or β-Catenin, neither in PANC1 (FIG. 7 middle panel) nor in A673 cells (FIG. 8 left panel). The genetic data was confirmed by chemical inhibition of DYRK1 using Harmine as described above. Like RNAi-mediated inhibition of DYRK1B, Harmine treatment of PANC1 and A673 reduced levels of GLI1 (FIGS. 7 and 8 right panels).

Example 3—DYRK1B Function is Essential for Tumor-Initiating Pancreatic Cancer Cells

The role of DYRK1B in GLI-dependent clonogenic growth of tumor-initiating cancer cells (PANC1 and L3.6p1 was addressed. An established 3D tumorsphere assay, suitable to monitor and quantify the growth of tumor-initiating pancreatic cancer stem cells under non-adherent conditions (see Eberl et al., 2012, incorporated herein by reference) was employed to analyze the effect of DYRK1B inhibition on the clonogenic growth properties of tumor-initiating pancreatic cancer cells. As shown in FIG. 9, RNAi mediated knockdown of DYRK1B, but not of DYRK1A, efficiently prevented clonogenic growth of tumor-initiating cells, in line with the results on the selective requirement of DYRK1B for GLI1 expression. In contrast the result obtained for the inhibition of DYRK1A, the pancreatic cancer stem cells do not form colonies upon inhibition of DYRK1B with shRNA, as evidenced by the lack of tumor cell spheroids. Like RNAi-mediated DYRK1B inhibition, Harmine treatment reduced clonogenic growth of PANC1 cells, whereas SMO-inhibition by GDC-0449 did not affect the growth of tumor-initiating cells (FIG. 10, FIG. 11). In the aforementioned experiments the viability of the cells was not affected by inhibition of DYRK1B (data not shown herein).

Example 4—Xenograft Assays

Xenograft assays with PANC1 and L3.6p1 cancer cells were prepared as described above. The PANC1 and L3.6p1 cancer cells were beforehand depleted of DYRK1B by DYRK1B shRNA transduction as described above. In agreement with the in vitro data, DYRK1B knockdown reduced in vivo growth of pancreatic cancer cells compared to control knockdown cells expressing non-target shRNA (FIG. 12, FIG. 13).

In summary, DYRK1B is a critical factor required for GLI1 expression in both SMO-dependent and SMO-independent human cancer cells, while DYRK1A function is largely dispensable for GLI1 expression. The essential requirement of DYRK1B in tumor-initiating pancreatic cells suggests that selective targeting of DYRK1B in HH/GLI-dependent human cancers is therefore a promising therapeutic approach, also effective in cells resistant to SMO-targeting such as pancreatic cancer and Ewing's sarcoma cells.

Example 5—In Vitro DYRK1A/DYRK1B Kinase Assay: Compound P

The assay was carried out by Reaction Biology Corp., Malverne, Pa., USA according to specifications by Reaction Biology Corp., as detailed herein below and further described in Anastassiadis et al., Nature Biotechnology, 29 (2011) 1039-1045.

DYRK1A: The substrate DYRKtide (synthetic peptide RRRFRPASPLRGPPK) (SEQ ID NO: 5) was dissolved in freshly prepared Base Reaction Buffer (20 mM Hepes (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 0.02% Brij35, 0.02 mg/ml BSA, 0.1 mM Na₃VO₄, 2 mM DTT, 1% DMSO) at a concentration of 20 μM. DYRK1A was added to the substrate solution in a concentration of 0.3 nM and gently mixed. Compound dilution series in DMSO were added to the reaction, followed 20 min later by addition of a mixture of ATP and ³³P ATP (0.01 μCi/μl final) to a final concentration of 10 μM. Reactions proceeded at 25° C. for 120 min, followed by spotting onto P81 ion exchange filter paper. Unbound phosphate was removed by extensive washing of the filters in 0.75% phosphoric acid. After subtraction of background derived from control reactions containing inactive enzyme, kinase activity data were expressed as the percent remaining kinase activity in test samples compared to vehicle (DMSO) reactions. IC₅₀ values and curve fits were obtained using Prism (Graph Pad Software).

The assay for DYRK1B was carried out as described above for DYRK1A, with the exception that instead of DYRK1A, DYRK1B was added to the substrate solution and gently mixed.

DYRK1A and DYRK1B used in the above assays are further described in the below table:

Genbank Protein Expres- Accession # Accession # Clone sion Tag DYRK1A NP_001387 Q13627 full-length Insect N-terminal GST DYRK1B NP_004705 Q9Y463 full-length Insect N-terminal GST

Compound dilution series which was applied for 4SC compound P, and which is exemplary for compound dilution series applicable in the above DYRK assays: DYRK1A: 3,00E-05 M, 1,00E-05 M, 3,33E-06 M, 1,11E-06 M, 3,70E-07 M, 1,23E-07 M, 4,12E-08 M, 1,37E-08 M, 4,57E-09 M, 1,52E-09 M; DYRK 1B: 1,00E-05 M, 3,33E-06 M, 1,11E-06 M, 3,70E-07 M, 1,23E-07 M, 4,12E-08 M, 1,37E-08 M, 4,57E-09 M, 1,52E-09 M, 5,08E-10 M.

In said assays, Compound P has an IC₅₀ of 22.2 nM (DYRK1A) and 54.8 nM (DYRK1B).

Example 6—DYRK1B Analogy Model

For identifying additional DYRK1B inhibitors a computational approach with the software 4SCan (for additional information and a detailed description of the method see: Seifert, H. J.; Wolf, K.; Vitt, D. Biosilico 2003, 1, 143 and/or Patent EP 1094 415 A2) was used. This iterative process starts by docking a small subset of a compound library into the target, in the present case DYRK1B. From the results a model for inhibitors based on molecular descriptors is generated and subsequently used to choose the next set of compounds for docking. The process is finished, after a predefined number of compounds are submitted to docking. 4SCan screening was conducted in 10 iterations docking 5,000 molecules each. Charged strong acids/bases were neutralized with the ‘wash’ function in the software MOE 2011.10 by adding/removing corresponding hydrogen atoms for the 2,000 top hits in order to convert the structure to a chemical name within ChemDrawExcel 12.0. Therefore a single compound name can exhibit multiple docking scores, depending on its protonation and tautomerization. The docking score calculated by the software ProPose (for additional information and a detailed description of the method see: Markus H. J. Seifert et al., Mol Model (2004) 10:342-357 is proportional to the change in Gibbs free energy upon ligand binding.

The 3D-structure of DYRK1B necessary for the aforementioned docking studies was not available from The Research Collaboratory for Structural Bioinformatics PDB (http://www.rcsb.org/pdb/) and was therefore generated by homology modeling using the closely related DYRK1A (G Manning et al. (2002) Science 298:1912-1934) as template. The template structure used is an X-ray structure of containing 4 representations (chains) of DYRK1A in complex with a ligand. The model is based on DYRK1A chain (A), wherein the side chain of Methionine 240 was remodeled to compensate for clashes with the ligand and because said Methionine conformation is different from the one in the other three chains (B-D) present in the X-ray structure.

Homology modeling (Jones, T. A. et al., EMBO J. 5 (1986) 819-822; Blundell T L et al. (1987) Nature 326(6111): 347-352) was carried out with the software MOE 2011.10, using the implemented approach with slightly varied standard parameters (Model refinement optimizations were set to ‘fine’ method). As DYRK1A and DYRK1B share 83.8% amino acid sequence homology, additional post-processing of the generated model was unnecessary.

The ligand binding site was determined by superposing the protein backbone of template and homology model with the software MOE 2011.10 and thus transferring the ligand of the DYRK1A X-ray structure to the homology model. Based on this ligand pose, the model used for ProPose docking in the 4SCan screening was generated by including all receptor atoms situated at a distance of 6.0 Å or less from at least one atom of the ligand, as potential interaction partners. The atom coordinates of the active site receptor atoms (6.0 Å radius) of the aforementioned model are shown below in pdb compatible format. All other atoms were omitted, as they are not essential to the modeling method as used herein because the active site conformations is kept constant in said method.

HEADER Q9Y463 DYRK1B_HUMAN 4SC Active Site Homology Model ATOM 1 CA ILE 117 46.444 22.603 −38.607 1.00 0.00 ATOM 2 C ILE 117 45.321 22.977 −39.591 1.00 0.00 ATOM 3 O ILE 117 44.878 22.154 −40.393 1.00 0.00 ATOM 4 CB ILE 117 46.012 21.520 −37.590 1.00 0.00 ATOM 5 CG1 ILE 117 45.028 22.039 −36.525 1.00 0.00 ATOM 6 CG2 ILE 117 47.257 20.892 −36.947 1.00 0.00 ATOM 7 CD1 ILE 117 44.383 20.924 −35.708 1.00 0.00 ATOM 8 HA ILE 117 47.202 22.143 −39.256 1.00 0.00 ATOM 9 HB ILE 117 45.503 20.724 −38.152 1.00 0.00 ATOM 10 HG12 ILE 117 45.536 22.723 −35.836 1.00 0.00 ATOM 11 HG13 ILE 117 44.226 22.604 −37.012 1.00 0.00 ATOM 12 HG21 ILE 117 47.950 20.529 −37.715 1.00 0.00 ATOM 13 HG22 ILE 117 47.794 21.617 −36.327 1.00 0.00 ATOM 14 HG23 ILE 117 46.996 20.033 −36.322 1.00 0.00 ATOM 15 HD11 ILE 117 43.874 20.206 −36.359 1.00 0.00 ATOM 16 HD12 ILE 117 45.119 20.385 −35.106 1.00 0.00 ATOM 17 HD13 ILE 117 43.640 21.341 −35.021 1.00 0.00 ATOM 18 N GLY 118 44.816 24.259 −39.464 1.00 0.00 ATOM 19 HA3 GLY 118 42.840 24.371 −40.075 1.00 0.00 ATOM 20 HA3 GLY 120 39.437 29.050 −42.282 1.00 0.00 ATOM 21 CB PHE 122 35.945 31.516 −39.362 1.00 0.00 ATOM 22 CG PHE 122 36.599 30.152 −39.232 1.00 0.00 ATOM 23 CD1 PHE 122 37.459 29.867 −38.161 1.00 0.00 ATOM 24 CD2 PHE 122 36.356 29.150 −40.181 1.00 0.00 ATOM 25 CE1 PHE 122 38.102 28.631 −38.070 1.00 0.00 ATOM 26 CE2 PHE 122 36.970 27.900 −40.070 1.00 0.00 ATOM 27 CZ PHE 122 37.852 27.645 −39.022 1.00 0.00 ATOM 28 HB1 PHE 122 35.620 31.836 −38.363 1.00 0.00 ATOM 29 HB2 PHE 122 35.024 31.412 −39.952 1.00 0.00 ATOM 30 HD1 PHE 122 37.637 30.611 −37.386 1.00 0.00 ATOM 31 HD2 PHE 122 35.668 29.323 −41.005 1.00 0.00 ATOM 32 HE1 PHE 122 38.777 28.429 −37.242 1.00 0.00 ATOM 33 HE2 PHE 122 36.744 27.120 −40.793 1.00 0.00 ATOM 34 HZ PHE 122 38.321 26.669 −38.937 1.00 0.00 ATOM 35 HA3 GLY 123 39.770 31.894 −37.417 1.00 0.00 ATOM 36 CB VAL 125 42.171 25.197 −36.304 1.00 0.00 ATOM 37 CG1 VAL 125 42.016 24.133 −35.210 1.00 0.00 ATOM 38 CG2 VAL 125 40.783 25.712 −36.698 1.00 0.00 ATOM 39 HB VAL 125 42.614 24.718 −37.186 1.00 0.00 ATOM 40 HG11 VAL 125 42.986 23.729 −34.905 1.00 0.00 ATOM 41 HG12 VAL 125 41.533 24.549 −34.319 1.00 0.00 ATOM 42 HG13 VAL 125 41.406 23.297 −35.566 1.00 0.00 ATOM 43 HG21 VAL 125 40.309 26.251 −35.870 1.00 0.00 ATOM 44 HG22 VAL 125 40.840 26.388 −37.557 1.00 0.00 ATOM 45 HG23 VAL 125 40.126 24.882 −36.981 1.00 0.00 ATOM 46 CD LYS 127 48.623 18.856 −33.368 1.00 0.00 ATOM 47 HD LYS 127 47.653 18.459 −33.691 1.00 0.00 ATOM 48 HB2 LYS 127 47.230 21.089 −33.947 1.00 0.00 ATOM 49 HE2 LYS 127 49.255 16.792 −33.199 1.00 0.00 ATOM 50 N ALA 138 43.208 21.773 −29.143 1.00 0.00 ATOM 51 CA ALA 138 43.020 22.343 −30.474 1.00 0.00 ATOM 52 C ALA 138 42.412 23.727 −30.262 1.00 0.00 ATOM 53 O ALA 138 41.276 23.894 −29.805 1.00 0.00 ATOM 54 CB ALA 138 42.078 21.495 −31.312 1.00 0.00 ATOM 55 H ALA 138 42.362 21.611 −28.570 1.00 0.00 ATOM 56 HA ALA 138 43.984 22.424 −30.986 1.00 0.00 ATOM 57 HB1 ALA 138 42.524 20.522 −31.521 1.00 0.00 ATOM 58 HB2 ALA 138 41.124 21.322 −30.803 1.00 0.00 ATOM 59 HB3 ALA 138 41.862 21.982 −32.269 1.00 0.00 ATOM 60 N LYS 140 41.179 27.330 −31.393 1.00 0.00 ATOM 61 CA LYS 140 40.558 28.079 −32.487 1.00 0.00 ATOM 62 CB LYS 140 39.067 27.736 −32.600 1.00 0.00 ATOM 63 CG LYS 140 38.280 28.451 −33.716 1.00 0.00 ATOM 64 CD LYS 140 36.793 28.031 −33.699 1.00 0.00 ATOM 65 CE LYS 140 35.949 28.688 −34.788 1.00 0.00 ATOM 66 NZ LYS 140 34.554 28.235 −34.711 1.00 0.00 ATOM 67 H LYS 140 40.639 27.249 −30.516 1.00 0.00 ATOM 68 HA LYS 140 41.064 27.832 −33.424 1.00 0.00 ATOM 69 HB2 LYS 140 38.977 26.652 −32.748 1.00 0.00 ATOM 70 HB3 LYS 140 38.588 27.962 −31.645 1.00 0.00 ATOM 71 HG2 LYS 140 38.351 29.536 −33.586 1.00 0.00 ATOM 72 HG3 LYS 140 38.723 28.206 −34.688 1.00 0.00 ATOM 73 HD2 LYS 140 36.364 28.271 −32.721 1.00 0.00 ATOM 74 HD3 LYS 140 36.734 26.941 −33.814 1.00 0.00 ATOM 75 HE2 LYS 140 36.314 28.393 −35.774 1.00 0.00 ATOM 76 HE3 LYS 140 35.942 29.779 −34.716 1.00 0.00 ATOM 77 HZ1 LYS 140 34.398 27.306 −34.315 1.00 0.00 ATOM 78 HZ2 LYS 140 33.844 28.848 −34.166 1.00 0.00 ATOM 79 HZ3 LYS 140 34.091 28.108 −35.696 1.00 0.00 ATOM 80 CD GLU 155 31.851 28.520 −33.176 1.00 0.00 ATOM 81 OE1 GLU 155 32.845 29.268 −33.442 1.00 0.00 ATOM 82 OE2 GLU 155 31.639 27.392 −33.707 1.00 0.00 ATOM 83 CD1 LEU 159 32.388 26.728 −29.358 1.00 0.00 ATOM 84 HD11 LEU 159 33.148 26.152 −28.828 1.00 0.00 ATOM 85 HD12 LEU 159 32.632 26.737 −30.423 1.00 0.00 ATOM 86 HD23 LEU 159 31.272 24.698 −30.743 1.00 0.00 ATOM 87 CB VAL 174 34.026 19.628 −30.894 1.00 0.00 ATOM 88 CG1 VAL 174 33.453 21.036 −31.057 1.00 0.00 ATOM 89 CG2 VAL 174 35.503 19.686 −30.501 1.00 0.00 ATOM 90 HB VAL 174 33.983 19.142 −31.876 1.00 0.00 ATOM 91 HG11 VAL 174 32.423 21.013 −31.423 1.00 0.00 ATOM 92 HG12 VAL 174 33.452 21.582 −30.112 1.00 0.00 ATOM 93 HG13 VAL 174 34.047 21.613 −31.773 1.00 0.00 ATOM 94 HG21 VAL 174 35.640 20.129 −29.512 1.00 0.00 ATOM 95 HG22 VAL 174 35.936 18.681 −30.482 1.00 0.00 ATOM 96 HG23 VAL 174 36.077 20.281 −31.218 1.00 0.00 ATOM 97 CD1 LEU 188 35.087 29.871 −30.220 1.00 0.00 ATOM 98 HD11 LEU 188 35.510 29.622 −31.195 1.00 0.00 ATOM 99 HD12 LEU 188 34.458 29.034 −29.898 1.00 0.00 ATOM 100 HB2 LEU 188 37.499 28.667 −30.023 1.00 0.00 ATOM 101 HB3 LEU 188 36.487 28.059 −28.746 1.00 0.00 ATOM 102 N PHE 190 39.437 23.853 −27.713 1.00 0.00 ATOM 103 CA PHE 190 38.715 22.593 −27.897 1.00 0.00 ATOM 104 C PHE 190 39.656 21.401 −27.640 1.00 0.00 ATOM 105 O PHE 190 40.887 21.468 −27.690 1.00 0.00 ATOM 106 CB PHE 190 38.196 22.432 −29.332 1.00 0.00 ATOM 107 CG PHE 190 37.193 23.463 −29.762 1.00 0.00 ATOM 108 CD1 PHE 190 35.825 23.260 −29.549 1.00 0.00 ATOM 109 CD2 PHE 190 37.621 24.621 −30.416 1.00 0.00 ATOM 110 CE1 PHE 190 34.894 24.161 −30.059 1.00 0.00 ATOM 111 CE2 PHE 190 36.684 25.531 −30.889 1.00 0.00 ATOM 112 CZ PHE 190 35.326 25.292 −30.740 1.00 0.00 ATOM 113 H PHE 190 40.182 24.026 −28.400 1.00 0.00 ATOM 114 HA PHE 190 37.894 22.547 −27.180 1.00 0.00 ATOM 115 HB2 PHE 190 39.035 22.439 −30.040 1.00 0.00 ATOM 116 HB3 PHE 190 37.742 21.441 −29.464 1.00 0.00 ATOM 117 HD1 PHE 190 35.469 22.390 −29.003 1.00 0.00 ATOM 118 HD2 PHE 190 38.680 24.812 −30.575 1.00 0.00 ATOM 119 HE1 PHE 190 33.832 23.974 −29.934 1.00 0.00 ATOM 120 HE2 PHE 190 37.010 26.428 −31.393 1.00 0.00 ATOM 121 HZ PHE 190 34.612 25.998 −31.146 1.00 0.00 ATOM 122 N GLU 191 38.969 20.205 −27.492 1.00 0.00 ATOM 123 CA GLU 191 39.667 18.923 −27.543 1.00 0.00 ATOM 124 C GLU 191 40.290 18.646 −28.936 1.00 0.00 ATOM 125 O GLU 191 39.723 18.913 −29.996 1.00 0.00 ATOM 126 CB GLU 191 38.746 17.767 −27.127 1.00 0.00 ATOM 127 CG GLU 191 37.523 17.546 −28.022 1.00 0.00 ATOM 128 H GLU 191 37.954 20.223 −27.335 1.00 0.00 ATOM 129 HA GLU 191 40.487 18.992 −26.818 1.00 0.00 ATOM 130 HB GLU 191 39.333 16.840 −27.087 1.00 0.00 ATOM 131 HG1 GLU 191 37.831 17.159 −28.999 1.00 0.00 ATOM 132 HG2 GLU 191 36.999 18.490 −28.192 1.00 0.00 ATOM 133 N LEU 192 41.536 18.024 −28.873 1.00 0.00 ATOM 134 CA LEU 192 42.277 17.645 −30.077 1.00 0.00 ATOM 135 C LEU 192 41.686 16.319 −30.579 1.00 0.00 ATOM 136 O LEU 192 41.266 15.439 −29.829 1.00 0.00 ATOM 137 CB LEU 192 43.779 17.517 −29.784 1.00 0.00 ATOM 138 CG LEU 192 44.676 17.299 −31.021 1.00 0.00 ATOM 139 CD1 LEU 192 44.642 18.482 −31.990 1.00 0.00 ATOM 140 CD2 LEU 192 46.120 17.042 −30.587 1.00 0.00 ATOM 141 H LEU 192 41.949 17.792 −27.973 1.00 0.00 ATOM 142 HA LEU 192 42.090 18.421 −30.823 1.00 0.00 ATOM 143 HB2 LEU 192 44.116 18.421 −29.259 1.00 0.00 ATOM 144 HB3 LEU 192 43.934 16.685 −29.083 1.00 0.00 ATOM 145 HG LEU 192 44.341 16.403 −31.556 1.00 0.00 ATOM 146 HD11 LEU 192 43.651 18.614 −32.432 1.00 0.00 ATOM 147 HD12 LEU 192 44.920 19.415 −31.488 1.00 0.00 ATOM 148 HD13 LEU 192 45.339 18.324 −32.819 1.00 0.00 ATOM 149 HD21 LEU 192 46.178 16.172 −29.926 1.00 0.00 ATOM 150 HD22 LEU 192 46.752 16.835 −31.456 1.00 0.00 ATOM 151 N LEU 193 41.659 16.224 −31.954 1.00 0.00 ATOM 152 CA LEU 193 40.998 15.164 −32.703 1.00 0.00 ATOM 153 C LEU 193 42.001 14.686 −33.774 1.00 0.00 ATOM 154 O LEU 193 43.192 15.009 −33.761 1.00 0.00 ATOM 155 CB LEU 193 39.706 15.730 −33.321 1.00 0.00 ATOM 156 CG LEU 193 38.657 16.223 −32.307 1.00 0.00 ATOM 157 CD1 LEU 193 37.487 16.875 −33.048 1.00 0.00 ATOM 158 CD2 LEU 193 38.143 15.094 −31.421 1.00 0.00 ATOM 159 H LEU 193 42.124 16.935 −32.508 1.00 0.00 ATOM 160 HA LEU 193 40.791 14.312 −32.048 1.00 0.00 ATOM 161 HB2 LEU 193 39.976 16.566 −33.980 1.00 0.00 ATOM 162 HB3 LEU 193 39.226 14.988 −33.963 1.00 0.00 ATOM 163 HG LEU 193 39.104 16.990 −31.667 1.00 0.00 ATOM 164 HD11 LEU 193 37.832 17.728 −33.640 1.00 0.00 ATOM 165 HD12 LEU 193 37.001 16.166 −33.726 1.00 0.00 ATOM 166 HD13 LEU 193 36.733 17.240 −32.344 1.00 0.00 ATOM 167 HD21 LEU 193 37.646 14.321 −32.015 1.00 0.00 ATOM 168 HD22 LEU 193 38.943 14.618 −30.850 1.00 0.00 ATOM 169 HD23 LEU 193 37.427 15.484 −30.694 1.00 0.00 ATOM 170 N SER 194 41.465 13.813 −34.705 1.00 0.00 ATOM 171 CA SER 194 42.273 13.215 −35.777 1.00 0.00 ATOM 172 C SER 194 42.120 14.067 −37.065 1.00 0.00 ATOM 173 O SER 194 42.012 15.294 −37.049 1.00 0.00 ATOM 174 CB SER 194 41.881 11.738 −35.933 1.00 0.00 ATOM 175 OG SER 194 42.606 11.082 −36.979 1.00 0.00 ATOM 176 H SER 194 40.464 13.574 −34.716 1.00 0.00 ATOM 177 HA SER 194 43.337 13.271 −35.515 1.00 0.00 ATOM 178 HB2 SER 194 40.823 11.633 −36.169 1.00 0.00 ATOM 179 HB3 SER 194 42.073 11.202 −34.998 1.00 0.00 ATOM 180 HG SER 194 42.337 10.145 −36.941 1.00 0.00 ATOM 181 N TYR 195 42.208 13.346 −38.241 1.00 0.00 ATOM 182 CA TYR 195 42.031 13.927 −39.578 1.00 0.00 ATOM 183 C TYR 195 40.535 14.271 −39.809 1.00 0.00 ATOM 184 O TYR 195 39.652 14.002 −38.987 1.00 0.00 ATOM 185 CB TYR 195 42.506 12.940 −40.664 1.00 0.00 ATOM 186 CG TYR 195 43.948 12.506 −40.526 1.00 0.00 ATOM 187 CD1 TYR 195 44.989 13.347 −40.941 1.00 0.00 ATOM 188 CD2 TYR 195 44.263 11.251 −39.984 1.00 0.00 ATOM 189 CE1 TYR 195 46.321 12.942 −40.809 1.00 0.00 ATOM 190 CE2 TYR 195 45.593 10.847 −39.847 1.00 0.00 ATOM 191 CZ TYR 195 46.614 11.698 −40.260 1.00 0.00 ATOM 192 OH TYR 195 47.927 11.334 −40.136 1.00 0.00 ATOM 193 H TYR 195 42.426 12.347 −38.149 1.00 0.00 ATOM 194 HA TYR 195 42.602 14.863 −39.618 1.00 0.00 ATOM 195 HB2 TYR 195 41.862 12.051 −40.660 1.00 0.00 ATOM 196 HB3 TYR 195 42.382 13.375 −41.660 1.00 0.00 ATOM 197 HD1 TYR 195 44.773 14.317 −41.384 1.00 0.00 ATOM 198 HD2 TYR 195 43.473 10.575 −39.666 1.00 0.00 ATOM 199 HE1 TYR 195 47.124 13.598 −41.134 1.00 0.00 ATOM 200 HE2 TYR 195 45.812 9.872 −39.424 1.00 0.00 ATOM 201 HH TYR 195 47.956 10.506 −39.633 1.00 0.00 ATOM 202 N ASN 196 40.248 14.910 −41.011 1.00 0.00 ATOM 203 H ASN 196 41.003 15.135 −41.681 1.00 0.00 ATOM 204 HB2 ASN 196 39.211 17.345 −41.043 1.00 0.00 ATOM 205 CB ASP 199 41.898 13.839 −44.603 1.00 0.00 ATOM 206 CG ASP 199 42.351 15.267 −44.399 1.00 0.00 ATOM 207 OD2 ASP 199 42.727 15.888 −45.449 1.00 0.00 ATOM 208 OD1 ASP 199 42.296 15.648 −43.194 1.00 0.00 ATOM 209 HB1 ASP 199 42.800 13.215 −44.656 1.00 0.00 ATOM 210 HB2 ASP 199 41.348 13.504 −43.719 1.00 0.00 ATOM 211 C GLU 243 35.270 20.258 −42.464 1.00 0.00 ATOM 212 O GLU 243 36.232 20.686 −41.824 1.00 0.00 ATOM 213 N ASN 244 33.990 20.196 −41.933 1.00 0.00 ATOM 214 CA ASN 244 33.636 20.686 −40.580 1.00 0.00 ATOM 215 C ASN 244 33.293 19.525 −39.609 1.00 0.00 ATOM 216 O ASN 244 32.827 19.694 −38.480 1.00 0.00 ATOM 217 CB ASN 244 32.457 21.671 −40.630 1.00 0.00 ATOM 218 CG ASN 244 32.859 23.064 −41.070 1.00 0.00 ATOM 219 OD1 ASN 244 34.018 23.460 −41.130 1.00 0.00 ATOM 220 ND2 ASN 244 31.803 23.864 −41.418 1.00 0.00 ATOM 221 HA ASN 244 34.519 21.170 −40.147 1.00 0.00 ATOM 222 HB2 ASN 244 31.999 21.785 −39.640 1.00 0.00 ATOM 223 HB3 ASN 244 31.676 21.296 −41.301 1.00 0.00 ATOM 224 HD2 ASN 244 31.914 24.813 −41.766 1.00 0.00 ATOM 225 CB LEU 246 37.350 16.327 −36.963 1.00 0.00 ATOM 226 CG LEU 246 38.272 17.316 −37.711 1.00 0.00 ATOM 227 CD1 LEU 246 37.498 18.328 −38.552 1.00 0.00 ATOM 228 CD2 LEU 246 39.171 18.051 −36.716 1.00 0.00 ATOM 229 HB1 LEU 246 36.718 16.877 −36.253 1.00 0.00 ATOM 230 HB2 LEU 246 37.988 15.672 −36.355 1.00 0.00 ATOM 231 HG LEU 246 38.926 16.745 −38.382 1.00 0.00 ATOM 232 HD11 LEU 246 37.009 17.848 −39.404 1.00 0.00 ATOM 233 HD12 LEU 246 36.730 18.825 −37.956 1.00 0.00 ATOM 234 HD13 LEU 246 38.165 19.095 −38.958 1.00 0.00 ATOM 235 HD21 LEU 246 38.582 18.626 −35.995 1.00 0.00 ATOM 236 HD22 LEU 246 39.794 17.341 −36.163 1.00 0.00 ATOM 237 HD23 LEU 246 39.844 18.743 −37.234 1.00 0.00 ATOM 238 O LEU 247 38.909 12.892 −35.280 1.00 0.00 ATOM 239 HB3 PRO 250 44.367 8.740 −37.457 1.00 0.00 ATOM 240 N VAL 258 32.839 20.006 −35.521 1.00 0.00 ATOM 241 CA VAL 258 33.562 21.186 −35.053 1.00 0.00 ATOM 242 C VAL 258 32.997 22.405 −35.785 1.00 0.00 ATOM 243 O VAL 258 32.550 22.327 −36.926 1.00 0.00 ATOM 244 CB VAL 258 35.095 21.095 −35.247 1.00 0.00 ATOM 245 CG1 VAL 258 35.680 19.850 −34.579 1.00 0.00 ATOM 246 CG2 VAL 258 35.535 21.135 −36.713 1.00 0.00 ATOM 247 H VAL 258 32.878 19.821 −36.532 1.00 0.00 ATOM 248 HA VAL 258 33.351 21.310 −33.986 1.00 0.00 ATOM 249 HB VAL 258 35.542 21.972 −34.759 1.00 0.00 ATOM 250 HG11 VAL 258 35.404 19.812 −33.524 1.00 0.00 ATOM 251 HG12 VAL 258 35.333 18.929 −35.058 1.00 0.00 ATOM 252 HG13 VAL 258 36.773 19.863 −34.632 1.00 0.00 ATOM 253 HG21 VAL 258 35.104 20.307 −37.280 1.00 0.00 ATOM 254 HG22 VAL 258 35.243 22.072 −37.198 1.00 0.00 ATOM 255 HG23 VAL 258 36.625 21.064 −36.789 1.00 0.00 ATOM 256 N ASP 259 33.076 23.590 −35.084 1.00 0.00 ATOM 257 CA ASP 259 32.516 24.861 −35.569 1.00 0.00 ATOM 258 C ASP 259 30.973 24.771 −35.585 1.00 0.00 ATOM 259 O ASP 259 30.355 23.808 −36.037 1.00 0.00 ATOM 260 CB ASP 259 33.035 25.335 −36.928 1.00 0.00 ATOM 261 CG ASP 259 32.659 26.812 −37.080 1.00 0.00 ATOM 262 OD1 ASP 259 33.553 27.633 −36.694 1.00 0.00 ATOM 263 OD2 ASP 259 31.491 27.050 −37.512 1.00 0.00 ATOM 264 H ASP 259 33.525 23.591 −34.173 1.00 0.00 ATOM 265 HA ASP 259 32.827 25.577 −34.804 1.00 0.00 ATOM 266 HB2 ASP 259 32.597 24.768 −37.757 1.00 0.00 ATOM 267 HB3 ASP 259 34.123 25.221 −36.990 1.00 0.00 ATOM 268 N PHE 260 30.358 25.863 −35.044 1.00 0.00 ATOM 269 H PHE 260 30.953 26.564 −34.526 1.00 0.00 ATOM 270 HD2 PHE 260 30.099 23.791 −33.004 1.00 0.00 ATOM 271 H GLY 261 30.374 27.597 −36.722 1.00 0.00 TER 272 GLY 261 END (Residues at positions 190-196 disclosed as SEQ ID NO: 6 and residues at positions 258-261 disclosed as SEQ ID NO: 7)

Table 1 shows the top hits found among a library of commercially available compounds with the above modeling approach, ranked by score/mol. wt. ratio (score/MW). While the ProPose Score is influenced by the size and thus the molecular weight of the tested compounds (because potential molecular interaction partners are more abundant in larger molecules), the score/molecular weight ratio generally represents a reasonable approach for identifying possible lead compounds. To maintain economical balance, Table 1 shows only every fifth hit. The results demonstrate that in terms of structure, a variety of small molecule inhibitors is accepted into the DYRK1B binding site and may be usable in the methods of the present invention. In the above modeling approach compound P has a score/weight ratio of −0.056 with a ProPose score of −15,803, thus showing a rationale for relating the score/weight ratio found in the above modeling approach to actual in vitro and in vivo binding affinity.

TABLE 1 Score/ ProPose Rank Chemical Name MW Score 1 coronene −0.106 −31.752 2 (Z)-3-(5-(2-cyano-2-(2-fluorophenyl)vinyl)furan-2-yl)benzoic acid −0.094 −31.269 3 10-(naphthalen-2-yl)-5H-benzo[c]furo[3,2-g]chromen-5-one −0.090 −32.709 4 (Z)-2-((5-(2-(trifluoromethyl)phenyl)furan-2- −0.090 −33.443 yl)methylene)benzo[b]thiophen-3(2H)-one 5 4-phenyl-2-(quinolin-2-yl)-10H-pyrido[2,3-b]carbazole −0.087 −36.672 6 (E)-3-(5-(2-(benzo[d]thiazol-2-yl)-2-cyanovinyl)furan-2-yl)benzoic acid −0.087 −32.212 7 perfluorophenyl 3-(thiazol-2-yl)benzoate −0.085 −31.555 8 9,10-di(naphthalen-2-yl)anthracene −0.085 −36.449 9 (Z)-2-(2-chloro-6-fluorobenzylidene)-3-oxo-2,3-dihydrobenzofuran-6-yl 2- −0.084 −34.682 fluorobenzoate 10 (Z)-2-chloro-5-(5-(2-(4-chlorophenyl)-2-cyanovinyl)furan-2-yl)benzoic acid −0.084 −32.066 15 (Z)-1-(5-((1-oxobenzo[4,5]imidazo[2,1-b]thiazol-2(1H)- −0.082 −38.926 ylidene)methyl)furan-2-yl)anthracene-9,10-dione 20 2-(5-methylthiophen-2-yl)-N-(perfluorophenyl)quinoline-4-carboxamide −0.081 −35.345 25 (Z)-5-((1H-indol-3-yl)methylene)-2-thioxo-3-(3- −0.080 −32.481 (trifluoromethyl)phenyl)thiazolidin-4-one 30 (E)-3-(5-((2-(2-(naphthalen-1-yl)acetyl)hydrazono)methyl)furan-2-yl)benzoic −0.080 −31.672 acid 35 3,4-bis(4-phenoxyphenyl)-2,5-diphenylcyclopenta-2,4-dienone −0.079 −45.185 40 (Z)-3-(5-((4-oxo-2-thioxo-3-(o-tolyl)thiazolidin-5-ylidene)methyl)furan-2- −0.079 −33.269 yl)benzoic acid 45 (Z)-3-benzyl-5-((5-(naphthalen-1-yl)furan-2-yl)methylene)-2- −0.079 −33.718 thioxothiazolidin-4-one 50 (Z)-5-(naphthalen-2-ylmethylene)-2-thioxo-3-(3- −0.079 −32.674 (trifluoromethyl)phenyl)thiazolidin-4-one 55 3-(benzofuran-2-carboxamido)-N-(3-chlorophenyl)benzofuran-2- −0.078 −33.630 carboxamide 60 3-(benzofuran-2-carboxamido)-N-(3-chloro-4-fluorophenyl)benzofuran-2- −0.078 −34.925 carboxamide 65 1-(4-phenoxyphenyl)-3-(quinolin-2-yl)benzo[f]quinoline −0.078 −36.803 70 5-(dibenzo[b,d]thiophen-2-yl)-2-(3-fluorophenyl)-4-phenyl-1H-imidazole −0.077 −32.521 75 ethyl 5-benzyl-2-(perfluorobenzamido)thiophene-3-carboxylate −0.077 −35.151 80 N-((8aR,10aR)-9,10-dioxo-8a,9,10,10a-tetrahydroanthracen-1-yl)-9-oxo-9H- −0.077 −33.185 fluorene-1-carboxamide 85 3-chloro-N-(2-fluoro-3-(trifluoromethyl)benzyl)naphtho[2,1-b]thiophene-2- −0.077 −33.503 carboxamide 90 N-(benzo[d][1,3]dioxol-5-yl)-3-(benzofuran-2-carboxamido)benzofuran-2- −0.076 −33.626 carboxamide 95 (E)-4-(5-((1-methyl-4,6-dioxo-3-phenyl-2-thioxotetrahydropyrimidin-5(2H)- −0.076 −32.857 ylidene)methyl)furan-2-yl)benzoic acid 100 N-(4-(2-oxo-1,2,3,4-tetrahydroquinolin-6-yl)thiazol-2-yl)-3- −0.076 −31.666 (trifluoromethyl)benzamide 105 5-((4-(benzyloxy)phenyl)amino)-3,8,10-trichloropyrene-1,6-dione −0.076 −40.303 110 (E)-3-oxo-4-(2-phenyl-1H-cyclopenta[b]chromen-4-ium-1-ylidene)-2-(2- −0.075 −42.695 phenylcyclopenta[b]chromen-1-yl)cyclobut-1-enolate 115 3-(5-cyano-1-cyclopropyl-6-oxo-1,6-dihydropyridin-3-yl)-N-(3,4- −0.075 −33.773 difluorophenyl)-5-fluorobenzofuran-2-carboxamide 120 2-((benzo[d]thiazol-2-ylmethyl)thio)-3-(2-fluorophenyl)quinazolin-4(3H)- −0.075 −31.411 one 125 (2Z,2′Z)-3,3′-(1,2-phenylene)bis(2-(naphthalen-1-yl)acrylonitrile) −0.075 −32.310 130 (E)-4-(5-((4-hydroxy-6-oxo-2-thioxo-1-(p-tolyl)-1,6-dihydropyrimidin-5(2H)- −0.075 −32.134 ylidene)methyl)furan-2-yl)benzoic acid 135 3-(5-((Z)-((Z)-2-((2,5-dimethylphenyl)imino)-4-oxothiazolidin-5- −0.074 −31.093 ylidene)methyl)furan-2-yl)benzoic acid 140 N-(5,7-difluorobenzo[d]thiazol-2-yl)-N-(furan-2-ylmethyl)benzo[d]thiazole- −0.074 −31.756 5-carboxamide 145 5-(dibenzo[b,d]thiophen-2-yl)-2-(3-fluorophenyl)-4-phenyl-1H-imidazole −0.074 −31.170 150 (E)-1-(((2-(2-benzylphenoxy)-5- −0.074 −36.731 (trifluoromethyl)phenyl)imino)methyl)naphthalen-2-ol 155 2-((1-oxo-2-phenyl-2,9-dihydro-1H-pyrimido[5,4-b]indol-3-yl)thio)-N-(3- −0.074 −36.536 (trifluoromethyl)phenyl)acetamide 160 (E)-2-cyano-3-(5-(3-nitrophenyl)thiophen-2-yl)-N-(2- −0.074 −32.697 (trifluoromethyl)phenyl)acrylamide 165 4-(4-(5-(3-(trifluoromethyl)phenyl)furan-2-carboxamido)benzyl)pyridin-1- −0.074 −31.169 ium 170 (E)-3-(4-chlorophenyl)-5-((5-(4-fluorophenyl)furan-2-yl)methylene)-6- −0.073 −31.279 hydroxy-2-thioxo-2,3-dihydropyrimidin-4(5H)-one 175 3-(naphthalen-2-yloxy)-4-oxo-2-(trifluoromethyl)-4H-chromen-7-yl 4- −0.073 −36.296 fluorobenzoate 180 (E)-2-(4-([1,1′-biphenyl]-4-yl)thiazol-2-yl)-3-(2-chloro-7-methoxyquinolin-3- −0.073 −35.173 yl)acrylonitrile 185 (Z)-3-cyano-N-(3-((2-fluorophenyl)carbamoyl)-2H-chromen-2-ylidene)- −0.073 −32.518 4,5,6,7-tetrahydrobenzo[b]thiophen-2-aminium 190 2,3,4,5,6-pentafluoro-N-(3-(2-oxo-2H-chromen-3-yl)phenyl)benzamide −0.073 −31.519 195 (E)-3-(5-(3,4-dichlorophenyl)furan-2-yl)-N-(2- −0.073 −31.127 (trifluoromethyl)phenyl)acrylamide 200 (E)-3-(5-(((3-cyano-4,5-bis(4-methoxyphenyl)furan-2-yl)imino)methyl)furan- −0.073 −37.734 2-yl)benzoic acid 205 2-(5-(4-chloro-3-(trifluoromethyl)phenyl)furan-2-yl)-6-fluoroquinoline-4- −0.073 −31.670 carboxylic acid 210 (Z)-2-((Z)-5-((5-(2-chlorophenyl)furan-2-yl)methylene)-4-oxo-3- −0.073 −38.373 phenylthiazolidin-2-ylidene)-2-cyano-N-(furan-2-ylmethyl)acetamide 215 2-(((1-(difluoromethyl)-1H-benzo[d]imidazol-2-yl)methyl)thio)-3-(2,4- −0.073 −34.160 difluorophenyl)quinazolin-4(3H)-one 220 N-(3-fluorophenyl)-2-((1-oxo-2-phenyl-1,2-dihydrobenzofuro[3,2- −0.073 −32.311 d]pyrimidin-3-yl)thio)acetamide 225 N-(2-fluorophenyl)-2-((1-oxo-2-phenyl-1,2-dihydrobenzofuro[3,2- −0.072 −32.284 d]pyrimidin-3-yl)thio)acetamide 230 3-([1,1′-biphenyl]-4-yloxy)-4-oxo-2-(trifluoromethyl)-4H-chromen-7-yl 3- −0.072 −38.834 chlorobenzoate 235 3-([1,1′-biphenyl]-4-yloxy)-7-((2-chlorobenzyl)oxy)-2-(trifluoromethyl)-4H- −0.072 −37.777 chromen-4-one 240 (E)-3-(naphthalen-1-yl)-2-(4-(3-oxo-3H-benzo[f]chromen-2-yl)thiazol-2- −0.072 −32.964 yl)acrylonitrile 245 (R) - N-(3-(3-(2,5-difluorophenyl)-4-oxothiazolidin-2-yl)phenyl)-2- −0.072 −33.182 naphthamide 250 (Z)-2-(2-chloro-6-fluorobenzylidene)-3-oxo-2,3-dihydrobenzofuran-6-yl 2- −0.072 −34.043 bromobenzoate 255 (E)-2-(4-(4-chlorophenyl)thiazol-2-yl)-3-(5-(3-nitrophenyl)furan-2- −0.072 −31.136 yl)acrylonitrile 260 2-(3-chlorophenyl)-5-(dibenzo[b,d]thiophen-2-yl)-4-phenyl-1H-imidazole −0.072 −31.298 265 2-(((6-chloroimidazo[1,2-a]pyridin-2-yl)methyl)thio)-3-(2,4- −0.072 −32.567 difluorophenyl)quinazolin-4(3H)-one 270 2-((1-oxo-2-phenyl-1,2-dihydrobenzofuro[3,2-d]pyrimidin-3-yl)thio)-N-(p- −0.072 −31.571 tolyl)acetamide 275 2-([1,1′-biphenyl]-4-yl)-N-(3-(trifluoromethyl)phenyl)quinoline-4- −0.071 −33.456 carboxamide 280 (E)-4-(5-((1-(3,5-dimethylphenyl)-4-hydroxy-6-oxo-2-thioxo-1,6- −0.071 −31.719 dihydropyrimidin-5(2H)-ylidene)methyl)furan-2-yl)benzoic acid 285 3-(benzofuran-2-carboxamido)-N-(2,3-dihydrobenzo[b][1,4]dioxin-6- −0.071 −32.403 yl)benzofuran-2-carboxamide 290 N-(3-(3H-benzo[d]imidazol-2-yl)-4-chlorophenyl)-2,3,4,5,6- −0.071 −31.194 pentafluorobenzamide 295 2-((3-((Z)-((Z)-4-oxo-3-phenyl-2-(phenylimino)thiazolidin-5-ylidene)methyl)- −0.071 −36.350 1H-indol-1-yl)methyl)benzonitrile 300 3-((2-(indolin-1-yl)-2-oxoethyl)thio)-2-(3-(trifluoromethyl)phenyl)-2,9- −0.071 −37.022 dihydro-1H-pyrimido[5,4-b]indol-1-one 305 (Z)-3-(2-((3-(2-((4-fluorophenyl)amino)-2-oxoethyl)-2,4-dioxothiazolidin-5- −0.071 −33.008 ylidene)methyl)-1H-pyrrol-1-yl)benzoic acid 310 (1Z,2E)-2-cyano-3-(5-(3-nitrophenyl)thiophen-2-yl)-N-(2- −0.071 −31.408 (trifluoromethyl)phenyl)acrylimidate 315 N-(2,4-difluorophenyl)-2-((2-(4-fluorophenyl)-1-oxo-2,9-dihydro-1H- −0.071 −34.057 pyrimido[5,4-b]indol-3-yl)thio)acetamide 320 3-((2-fluorobenzyl)thio)-2,6-diphenylthieno[2,3-d]pyrimidin-1(2H)-one −0.071 −31.474 325 3-((2-(3,4-difluorophenyl)-2-oxoethyl)thio)-7-(furan-2-yl)-2- −0.071 −33.993 phenylthieno[2,3-d]pyrimidin-1(2H)-one 330 (R)-2-(naphthalen-1-yl)-14-phenyl-14H-benzo[5,6]chromeno[3,2- −0.071 −33.675 e][1,2,4]triazolo[1,5-c]pyrimidine 335 (Z)-2-benzyl-6-((5-(3-(trifluoromethyl)phenyl)furan-2-yl)methylene)-3H- −0.071 −33.992 thiazolo[3,2-b][1,2,4]triazine-3,7(6H)-dione 340 2-((3-(2,4-difluorophenyl)-4-oxo-3,4-dihydroquinazolin-2-yl)thio)-N- −0.071 −33.406 (naphthalen-1-yl)acetamide 345 3-((4-fluorobenzyl)thio)-2,6-diphenylthieno[2,3-d]pyrimidin-1(2H)-one −0.071 −31.342 350 3-((2-oxo-2-phenylethyl)thio)-2,6-diphenylthieno[2,3-d]pyrimidin-1(2H)-one −0.070 −31.985 355 N-(2-benzoyl-4-chlorophenyl)-2-((4-phenylquinazolin-2-yl)thio)acetamide −0.070 −35.849 360 (Z)-3-benzyl-5-((5-(2,4-dichlorophenyl)furan-2-yl)methylene)-2- −0.070 −31.356 thioxothiazolidin-4-one 365 (1-amino-5,7-bis(4-methoxyphenyl)thieno[2,3-b]pyridin-2-yl)(naphthalen-2- −0.070 −36.259 yl)methanone 370 (Z)-2-((4-chlorobenzyl)thio)-1-(4-chlorophenyl)-4-(4-fluorobenzylidene)-1H- −0.070 −32.080 imidazol-5(4H)-one 375 (Z) - N-(3-(1H-benzo[d]imidazol-2-yl)-6,8-dichloro-2H-chromen-2-ylidene)- −0.070 −33.879 [1,1′-biphenyl]-4-aminium 380 N-(2-chloro-5-(6-methylbenzo[d]oxazol-2-yl)phenyl)-2,3,4,5,6- −0.070 −31.710 pentafluorobenzamide 385 (E) - N-(2-(2-chloro-4,5-difluorophenyl)benzo[d]oxazol-5-yl)-3-(4- −0.070 −31.164 chlorophenyl)acrylamide 390 N-(4-chloro-2-(2-chlorobenzoyl)phenyl)-5-(thiophen-2-yl)-7- −0.070 −39.252 (trifluoromethyl)pyrazolo[1,5-a]pyrimidine-2-carboxamide 395 3-(2,4-difluorophenyl)-2-((2-(indolin-1-yl)-2-oxoethyl)thio)quinazolin-4(3H)- −0.070 −31.389 one 400 (Z)-2-benzyl-5-((5-(3-(trifluoromethyl)phenyl)furan-2- −0.070 −31.635 yl)methylene)thiazolo[3,2-b][1,2,4]triazol-6(5H)-one 405 2-(4-(benzyloxy)phenyl)-5-(dibenzo[b,d]thiophen-2-yl)-4-phenyl-1H- −0.070 −35.456 imidazole 410 (4-(2-([1,1′-biphenyl]-4-yl)-5-phenyl-1H-imidazol-4- −0.070 −33.183 yl)phenyl)(phenyl)methanone 415 (2-amino-1-(4-(4-nitrophenyl)thiazol-2-yl)indolizin-3-yl)(4- −0.070 −31.910 fluorophenyl)methanone 420 3-(naphthalen-2-yloxy)-4-oxo-2-(trifluoromethyl)-4H-chromen-7-yl 2- −0.070 −35.216 methoxybenzoate 425 (Z)-4-chloro-3-(5-((3-(2-chloro-6-fluorobenzyl)-2,4-dioxothiazolidin-5- −0.069 −34.113 ylidene)methyl)furan-2-yl)benzoic acid 430 2,2′-((4-methoxyphenyl)methylene)bis(1H-phenalene-1,3(2H)-dione) −0.069 −35.421 435 N,N′-(1,2-phenylene)bis(5-(4-fluorophenyl)furan-2-carboxamide) −0.069 −33.557 440 3-(benzofuran-2-carboxamido)-N-(2,4-dimethoxyphenyl)benzofuran-2- −0.069 −31.582 carboxamide 445 (E)-3-(3-(2-((1-naphthoyl)oxy)phenyl)-2-cyanoacrylamido)benzoic acid −0.069 −31.906 450 2-(4-fluorophenyl)-3-((2-(indolin-1-yl)-2-oxoethyl)thio)-2,9-dihydro-1H- −0.069 −32.460 pyrimido[5,4-b]indol-1-one 455 2-chloro-4-(5-((Z)-((Z)-3-(2-methoxyethyl)-4-oxo-2-(phenylimino)thiazolidin- −0.069 −33.229 5-ylidene)methyl)furan-2-yl)benzoic acid 460 2-((3-(4-ethoxyphenyl)-4-oxo-3,4-dihydroquinazolin-2-yl)thio)-N-(3- −0.069 −34.421 (trifluoromethyl)phenyl)acetamide 465 (Z)-3-((2-chloro-5-(trifluoromethyl)phenyl)amino)-2-(4-(2-oxo-2H-chromen- −0.069 −32.616 3-yl)thiazol-2-yl)acrylonitrile 470 N-(9,10-dioxo-9,10-dihydroanthracen-1-yl)-2-((4-(4-fluorophenyl)-1H- −0.069 −31.454 imidazol-2-yl)thio)acetamide 475 N-(5-chloro-2-fluorophenyl)-2-((3-(2-chlorophenyl)-4-oxo-3,4- −0.069 −32.553 dihydroquinazolin-2-yl)thio)acetamide 480 3-((2-(4-fluorophenyl)-2-oxoethyl)thio)-10-oxo-11-phenethyl-6,7,8,9,10.11- −0.069 −35.633 hexahydrobenzo[4,5]thieno[3,2-e][1,2,4]triazolo[4,3-a]pyrimidin-2-ium 485 (Z)-5-(5-chloro-2-((2-fluorobenzyl)oxy)benzylidene)-3-(3-fluorophenyl)-2- −0.068 −32.466 thioxothiazolidin-4-one 490 2-((2-(4-chlorophenyl)-1-oxo-2,9-dihydro-1H-pyrimido[5,4-b]indol-3- −0.068 −33.999 yl)thio)-N-(2,4-difluorophenyl)acetamide 495 (Z)-2-(4-bromobenzoyl)-8-(furan-2-yl)-5-(furan-2-ylmethylene)-6,7-dihydro- −0.068 −36.022 5H-cyclopenta[b]thieno[3,2-e]pyridine-1-carbonitrile 500 (R)-2-((((9H-fluoren-9-yl)methoxy)carbonyl)amino)-3- −0.068 −32.509 (perfluorophenyl)propanoic acid 505 10-(2-chloro-4-fluorobenzyl)-N-(3-(3,4-dihydroisoquinolin-2(1H)-yl)propyl)- −0.068 −40.007 11-oxo-10.11-dihydrodibenzo[b,f][1,4]thiazepine-8-carboxamide 510 N-(3,4-difluorophenyl)-2-((3-(2,4-difluorophenyl)-4-oxo-3,4- −0.068 −31.281 dihydroquinazolin-2-yl)thio)acetamide 515 N2,N5-bis(1,2-dihydroacenaphthylen-6-yl)thiophene-2,5-dicarboxamide −0.068 −32.291 520 (2Z,5Z)-5-((5-(2-bromo-4-methylphenyl)furan-2-yl)methylene)-3-(furan-2- −0.068 −35.336 ylmethyl)-2-(phenylimino)thiazolidin-4-one 525 3-(2-((2-fluorobenzyl)thio)-5-(phenylthio)pyrimidine-4- −0.068 −33.343 carboxamido)benzoic acid 530 (E)-4-(4-(diphenylamino)benzylidene)-1,2,3,4-tetrahydroacridin-10-ium-9- −0.068 −32.777 carboxylate 535 N-(3,4-dichlorophenyl)-2-((1-oxo-2-phenyl-2,9-dihydro-1H-pyrimido[5,4- −0.068 −33.627 b]indol-3-yl)thio)acetamide 540 N-(2-chloro-5-(4,6-dimethylbenzo[d]oxazol-2-yl)phenyl)-2,3,4,5,6- −0.068 −31.675 pentafluorobenzamide 545 N-(2-ethylphenyl)-2-((1-oxo-2-(p-tolyl)-1,2-dihydrobenzofuro[3,2- −0.068 −31.851 d]pyrimidin-3-yl)thio)acetamide 550 4-(5-((Z)-((Z)-3-(2-(1H-indol-3-yl)ethyl)-2-((4-methoxyphenyl)imino)-4- −0.068 −36.900 oxothiazolidin-5-ylidene)methyl)furan-2-yl)benzonitrile 555 N-(2-(phenylcarbamoyl)benzofuran-3-yl)-9H-xanthene-9-carboxamide −0.068 −31.185 560 9-(phenethylthio)-8-(m-tolyl)-5,6-dihydronaphtho[2′,1′:4,5]thieno[2,3- −0.068 −32.533 d]pyrimidin-7(8H)-one 565 N-(4-fluorobenzyl)-2-((2-(4-fluorophenyl)-1-oxo-2,9-dihydro-1H- −0.068 −32.216 pyrimido[5,4-b]indol-3-yl)thio)acetamide 570 (Z)-2-cyano-2-((Z)-5-((5-(2-nitrophenyl)furan-2-yl)methylene)-4-oxo-3- −0.068 −36.119 phenylthiazolidin-2-ylidene)-N-phenylacetamide 575 2-(3-chloro-4-((2-fluorobenzyl)oxy)-5-methoxyphenyl)-5,6,7,8- −0.068 −31.810 tetrahydrobenzo[4,5]thieno[2,3-d]pyrimidin-4(3H)-one 580 (R)-ethyl 2-(perfluorobenzamido)-6-phenyl-4,5,6,7- −0.067 −33.434 tetrahydrobenzo[b]thiophene-3-carboxylate 585 2-phenyl-5-(m-tolylamino)-3-(p-tolylthio)-1H-naphtho[2,3-g]indole-6,11- −0.067 −37.131 dione 590 (Z)-4-(5-((4-oxo-2-thioxo-3-(3-(trifluoromethyl)phenyl)thiazolidin-5- −0.067 −31.977 ylidene)methyl)furan-2-yl)benzoic acid 595 2-((2,6-diphenyl-4H-pyran-4-ylidene)methyl)-4,6-diphenylthiopyrylium −0.067 −33.244 600 3-([1,1′-biphenyl]-4-yloxy)-7-((2,3,6-trichlorobenzyl)oxy)-2- −0.067 −39.843 (trifluoromethyl)-4H-chromen-4-one 605 N-(3-fluorophenyl)-2-((4-oxo-3-(4-phenoxyphenyl)-3,4-dihydroquinazolin-2- −0.067 −33.445 yl)thio)acetamide 610 N-(3,4-difluorophenyl)-3-(2,2-diphenylacetamido)benzofuran-2- −0.067 −32.403 carboxamide 615 2-(5-(2-chlorophenyl)furan-2-yl)-N-(4-(trifluoromethyl)phenyl)quinoline-4- −0.067 −33.082 carboxamide 620 6-(4-((4-fluorobenzyl)oxy)phenyl)-2-((4-fluorobenzyl)thio)-4- −0.067 −34.367 (trifluoromethyl)nicotinonitrile 625 (Z)-3-(5-((3-(3,4-dichlorophenyl)-4-oxo-2-thioxothiazolidin-5- −0.067 −31.848 ylidene)methyl)furan-2-yl)benzoic acid 630 5-(3-nitrophenyl)-N-((2-(pyridin-4-yl)benzo[d]oxazol-6- −0.067 −32.493 yl)carbamothioyl)furan-2-carboxamide 635 4-(((3-(2-chloro-5-(trifluoromethyl)phenyl)-4-oxo-3,4-dihydroquinazolin-2- −0.067 −31.554 yl)thio)methyl)benzonitrile 640 (E)-5-((5-(3-nitrophenyl)furan-2-yl)methylene)-2-thioxo-3-(3- −0.067 −31.821 (trifluoromethyl)phenyl)thiazolidin-4-one 645 (E)-3-(5-(3-nitrophenyl)furan-2-yl)-2-(4-(2-oxo-2H-chromen-3-yl)thiazol-2- −0.067 −31.196 yl)acrylonitrile 650 N-(4-bromo-2-(2-fluorobenzoyl)phenyl)-5-(thiophen-2-yl)-7- −0.067 −39.313 (trifluoromethyl)pyrazolo[1,5-a]pyrimidine-2-carboxamide 655 (E)-3-(5-((3-(2-chloro-6-fluorobenzyl)-2,4-dioxothiazolidin-5- −0.067 −31.375 ylidene)methyl)furan-2-yl)-4-methylbenzoic acid 660 (E)-3-((3-((2-((4-fluorophenyl)amino)-4-oxothiazol-5(4H)-ylidene)methyl)- −0.067 −31.323 1H-indol-1-yl)methyl)benzoic acid 665 (Z)-5-(3-(benzyloxy)benzylidene)-2-thioxo-3-(3- −0.067 −31.372 (trifluoromethyl)phenyl)thiazolidin-4-one 670 (carbonylbis(4,1-phenylene))bis((1,2-dihydroacenaphthylen-6- −0.066 −36.082 yl)methanone) 675 (3R,3aR,6aS)-5-(4-chlorophenyl)-3-(naphthalen-1-yl)-3,3a- −0.066 −33.759 dihydrospiro[furo[3,4-c]pyrrole-1,2′-indene]-1′,3′,4,6(5H,6aH)-tetraone 680 3-(2-chloro-5-(trifluoromethyl)phenyl)-4-oxo-N-(4-phenoxyphenyl)-2- −0.066 −37.711 thioxo-1,2,3,4-tetrahydroquinazoline-7-carboxamide 685 N-(3-chloro-4-fluorophenyl)-2-((2-(4-fluorophenyl)-1-oxo-1,2- −0.066 −33.052 dihydrobenzofuro[3,2-d]pyrimidin-3-yl)thio)acetamide 690 3-((2-(naphthalen-2-yl)-2-oxoethyl)thio)-2,6-diphenylthieno[2,3- −0.066 −33.487 d]pyrimidin-1(2H)-one 695 (Z)-4-(5-((1-(4-bromophenyl)-2-hydroxy-4,6-dioxo-1,6-dihydropyrimidin- −0.066 −31.789 5(4H)-ylidene)methyl)furan-2-yl)benzoic acid 700 (R) - N-(3,4-difluorophenyl)-2-(5-(2-(trifluoromethyl)phenyl)benzo[d]oxazol- −0.066 −32.317 2-yl)pyrrolidine-1-carboxamide 705 (R)-2-((3-(2,4-difluorophenyl)-4-oxo-3,4-dihydroquinazolin-2-yl)thio)-N-(1- −0.066 −31.111 (4-fluorophenyl)ethyl)acetamide 710 5-(4-((2-chlorobenzyl)oxy)benzylidene)-1,3-diphenyl-2- −0.066 −34.781 thioxodihydropyrimidine-4,6(1H,5H)-dione 715 3-(2-(6-methylbenzofuran-3-yl)acetamido)-N-(3- −0.066 −32.612 (trifluoromethyl)phenyl)benzofuran-2-carboxamide 720 (Z)-4-chloro-3-(5-((3-(2-((2,3-dihydrobenzo[b][1,4]dioxin-7-yl)amino)-2- −0.066 −35.710 oxoethyl)-2,4-dioxothiazolidin-5-ylidene)methyl)furan-2-yl)benzoic acid 725 N-(2-fluorophenyl)-2-((4-oxo-3-(4-phenoxyphenyl)-3,4-dihydroquinazolin-2- −0.066 −32.875 yl)thio)acetamide 730 1,4-bis(2-(4-fluorophenyl)-4-phenyl-1H-imidazol-5-yl)benzene −0.066 −36.371 735 N-(3-cyano-5,6,7,8-tetrahydro-4H-cyclohepta[b]thiophen-2-yl)-5-(furan-2- −0.066 −31.735 yl)-1-phenylisoxazolo[5,4-b]pyridine-7-carboxamide 740 N-(3-(6-(2,3-dihydrobenzo[b][1,4]dioxin-7-yl)pyridin-2-yl)phenyl)-2,3,4,5,6- −0.066 −32.883 pentafluorobenzamide 745 (2Z,5Z)-2-((4-chloro-3-(trifluoromethyl)phenyl)imino)-5-(4-((2- −0.066 −34.516 chlorobenzyl)oxy)benzylidene)thiazolidin-4-one 750 2-((E)-((4-(2-(4-((E)-(2-hydroxybenzylidene)amino)phenyl)quinazolin-4- −0.066 −34.256 yl)phenyl)imino)methyl)phenolate 755 (Z)-5-((5-(3-nitrophenyl)furan-2-yl)methylene)-2-thioxo-3-(3- −0.066 −31.396 (trifluoromethyl)phenyl)thiazolidin-4-one 760 (S) - N-(1-cyano-5-methyl-4,5,6,7-tetrahydrobenzo[b]thiophen-2-yl)-5-(furan- −0.066 −31.639 2-yl)-1-phenylisoxazolo[5,4-b]pyridine-7-carboxamide 765 N-(3-chloro-4-fluorophenyl)-3-(3-(trifluoromethyl)benzamido)benzofuran- −0.066 −31.368 2-carboxamide 770 (2Z,5Z)-5-(4-((2-chlorobenzyl)oxy)-3-ethoxybenzylidene)-2-((2- −0.066 −31.753 fluorophenyl)imino)thiazolidin-4-one 775 N-(3,5-dimethylphenyl)-2-((2-(4-fluorophenyl)-1-oxo-1,2- −0.066 −31.104 dihydrobenzofuro[3,2-d]pyrimidin-3-yl)thio)acetamide 780 2-((3-(2,4-difluorophenyl)-4-oxo-3,4-dihydroquinazolin-2-yl)thio)-N-(2-(2- −0.066 −32.650 methoxyphenoxy)ethyl)acetamide 785 (Z)-5-((5-(2-chloro-5-nitrophenyl)furan-2-yl)methylene)-2-thioxo-3-(3- −0.066 −33.525 (trifluoromethyl)phenyl)thiazolidin-4-one 790 6-chloro-N-(4-chlorophenyl)-2-(5-(3-(trifluoromethyl)phenyl)furan-2- −0.066 −32.094 yl)imidazo[1,2-a]pyridin-1-amine 795 N-(naphthalen-1-yl)-2-((4-oxo-3-phenyl-3,4,6,7,8,9-hexahydropyrimido[4,5- −0.066 −32.283 b]quinolin-2-yl)thio)acetamide 800 2-((3-(4-(difluoromethoxy)phenyl)-4-oxo-3,4-dihydroquinazolin-2-yl)thio)-N- −0.065 −32.036 (2,4-difluorophenyl)acetamide 805 2-(3-bromophenyl)-N-(perfluorophenyl)quinoline-4-carboxamide −0.065 −32.279 810 2-((E)-((4-(4-(4-((E)-(2-hydroxybenzylidene)amino)phenyl)quinazolin-2- −0.065 −33.986 yl)phenyl)imino)methyl)phenolate 815 N-([1,1′-biphenyl]-2-yl)-2-((3-(2,4-difluorophenyl)-4-oxo-3,4- −0.065 −32.659 dihydroquinazolin-2-yl)thio)acetamide 820 (3-(9-chloro-5H-chromeno[4,3-b]pyridin-2-yl)-5-methylbenzofuran-2-yl)(4- −0.065 −31.746 chlorophenyl)methanone 825 3-(2-([1,1′-biphenyl]-4-yl)acetamido)-N-(3,4-difluorophenyl)benzofuran-2- −0.065 −31.476 carboxamide 830 (Z)-5-((5-(4-chloro-3-(trifluoromethyl)phenyl)furan-2-yl)methylene)-3-(2- −0.065 −32.069 oxo-2-phenylethyl)thiazolidine-2,4-dione 835 (Z)-ethyl 2-(4-(((2,6-difluorophenyl)amino)methylene)-1,3-dioxo-3,4- −0.065 −33.131 dihydroisoquinolin-2(1H)-yl)-4,5,6,7-tetrahydrobenzo[b]thiophene-3- carboxylate 840 N-(2-((3,4-difluorophenyl)carbamoyl)benzofuran-3-yl)-9H-xanthene-9- −0.065 −32.329 carboxamide 845 8-benzyl-9-(phenethylthio)-5,6-dihydronaphtho[2′,1′:4,5]thieno[2,3- −0.065 −31.280 d]pyrimidin-7(8H)-one 850 2-((E)-3-((E)-(3-((E)-2-(quinolin-2-yl)vinyl)phenyl)diazenyl)styryl)quinolin-1- −0.065 −31.838 ium 855 N-(9,10-dioxo-9,10-dihydroanthracen-2-yl)-3-(indolin-1- −0.065 −33.023 ylsulfonyl)benzamide 860 (R,Z)-7-(4-chlorophenyl)-10-((5-(3-(trifluoromethyl)phenyl)furan-2- −0.065 −38.232 yl)methylene)-7,10-dihydro-5H-benzo[h]thiazolo[2,3-b]quinazolin-9(6H)- one 865 N-(2-ethoxyphenyl)-2-((2-(4-fluorophenyl)-1-oxo-2,9-dihydro-1H- −0.065 −31.687 pyrimido[5,4-b]indol-3-yl)thio)acetamide 870 methyl 2-(5-(furan-2-yl)-7-(trifluoromethyl)pyrazolo[1,5-a]pyrimidine-2- −0.065 −31.799 carboxamido)-4,5,6,7-tetrahydrobenzo[b]thiophene-1-carboxylate 875 (2Z,5E)-2-((2-chloro-5-(trifluoromethyl)phenyl)imino)-5-((5-(3- −0.065 −32.009 nitrophenyl)furan-2-yl)methylene)thiazolidin-4-one 880 (Z)-3-(2-((3-(2-((4-chlorophenyl)amino)-2-oxoethyl)-2,4-dioxothiazolidin-5- −0.065 −31.154 ylidene)methyl)-1H-pyrrol-1-yl)benzoic acid 885 (Z)-2-((3-(2-oxo-2H-chromen-3-yl)-1-phenyl-1H-pyrazol-4- −0.065 −31.635 yl)methylene)benzo[4,5]imidazo[2,1-b]thiazol-3(2H)-one 890 N-(3-chloro-4-fluorophenyl)-4-oxo-2-thioxo-3-(3-(trifluoromethyl)phenyl)- −0.065 −31.960 1,2,3,4-tetrahydroquinazoline-7-carboxamide 895 6-(4-((2,6-dichlorobenzyl)oxy)phenyl)-3-methyl-1-phenyl-4- −0.065 −34.175 (trifluoromethyl)-1H-pyrazolo[3,4-b]pyridine 900 N-(4-chloro-3-(trifluoromethyl)phenyl)-7-(chlorodifluoromethyl)-5- −0.065 −32.799 (thiophen-2-yl)pyrazolo[1,5-a]pyrimidine-2-carboxamide 905 2-((2-([1,1′-biphenyl]-4-yl)-2-oxoethyl)thio)-4-(4-fluorophenyl)-6- −0.065 −32.349 phenylnicotinonitrile 910 (Z)-2-(4-oxo-2-thioxo-5-(2-(trifluoromethyl)benzylidene)thiazolidin-3-yl)-1H- −0.065 −31.287 benzo[de]isoquinoline-1,3(2H)-dione 915 2-((2-(4-ethoxyphenyl)-1-oxo-2,9-dihydro-1H-pyrimido[5,4-b]indol-3- −0.065 −31.521 yl)thio)-N-(3-fluorophenyl)acetamide 920 4-((1-oxo-3-((2-(trifluoromethyl)benzyl)thio)-6,7,8,9- −0.064 −31.500 tetrahydrobenzo[4,5]thieno[2,3-d]pyrimidin-2(1H)-yl)methyl)pyridin-1-ium 925 2-((2-(4-fluorobenzyl)-1-oxo-1,2-dihydrobenzofuro[3,2-d]pyrimidin-3- −0.064 −31.525 yl)thio)-N-(3-methoxyphenyl)acetamide 930 (R)-2-((1-oxo-2-(3-(trifluoromethyl)phenyl)-2,9-dihydro-1H-pyrimido[5,4- −0.064 −32.335 b]indol-3-yl)thio)-N-((tetrahydrofuran-2-yl)methyl)acetamide 935 (Z)-2-((6-((5-chloro-2-hydroxybenzylidene)amino)benzo[d]thiazol-2-yl)thio)- −0.064 −34.921 N-(dibenzo[b,d]furan-3-yl)acetamide 940 6-chloro-3-(2,4-difluorophenyl)-2-((2-(3,4-dihydroisoquinolin-2(1H)-yl)-2- −0.064 −31.992 oxoethyl)thio)quinazolin-4(3H)-one 945 (Z)-3-(5-((7-imino-5-oxo-2-((o-tolyloxy)methyl)-5H-[1,3,4]thiadiazolo[3,2- −0.064 −31.174 a]pyrimidin-6(7H)-ylidene)methyl)furan-2-yl)benzoic acid 950 (2Z,5Z)-3-phenyl-2-(phenylimino)-5-((5-(3-(trifluoromethyl)phenyl)furan-3- −0.064 −31.467 yl)methylene)thiazolidin-4-one 955 (E)-5-((1-(2-chlorobenzyl)indolin-5-yl)methylene)-6-hydroxy-3-(naphthalen- −0.064 −33.525 1-yl)-2-thioxo-2,3-dihydropyrimidin-4(5H)-one 960 (Z)-1-(2,4-dichlorobenzoyl)-3-(((2,4-dichlorobenzoyl)oxy)imino)indolin-2- −0.064 −32.562 one 965 2-(2-(1H-indol-3-yl)ethyl)-3-((2-fluorobenzyl)thio)benzo[4,5]thieno[3,2- −0.064 −31.092 d]pyrimidin-1(2H)-one 970 2-chloro-5-(5-((Z)-((Z)-4-oxo-3-phenyl-2-(phenylimino)thiazolidin-5- −0.064 −32.004 ylidene)methyl)furan-2-yl)benzoic acid 975 N-(3-methoxyphenyl)-2-((2-(4-methoxyphenyl)-1-oxo-2,9-dihydro-1H- −0.064 −31.118 pyrimido[5,4-b]indol-3-yl)thio)acetamide 980 9-((2-(4-chlorophenyl)-2-oxoethyl)thio)-8-phenyl-5,6- −0.064 −32.913 dihydronaphtho[2′,1′:4,5]thieno[2,3-d]pyrimidin-7(8H)-one 985 5-(2-(acridin-10-ium-3-yl)-1,3-dioxoisoindoline-5-carbonyl)-1-oxo-1H- −0.064 −31.840 isoindol-3-olate 990 ethyl 4-(2-((3-(4-fluorophenyl)-4-oxo-4,5-dihydro-3H-pyrimido[5,4-b]indol- −0.064 −32.951 2-yl)thio)acetamido)benzoate 995 methyl 3-((6-chloro-2-(5-(3-(trifluoromethyl)phenyl)furan-2-yl)imidazo[1,2- −0.064 −32.703 a]pyridin-1-yl)amino)benzoate 1000 2-(benzylthio)-6-(4-((4-fluorobenzyl)oxy)phenyl)-4- −0.064 −31.494 (trifluoromethyl)nicotinonitrile 1005 N-(2-ethoxyphenyl)-2-((2-(4-fluorophenyl)-1-oxo-1,2-dihydrobenzofuro[3,2- −0.064 −31.140 d]pyrimidin-3-yl)thio)acetamide 1010 (Z)-ethyl 3-(((1,3-dioxo-2-(p-tolyl)-2,3-dihydroisoquinolin-4(1H)- −0.064 −33.459 ylidene)methyl)amino)-5-(4-fluorophenyl)thiophene-2-carboxylate 1015 3-((2-(4-chlorophenyl)-2-oxoethyl)thio)-10-oxo-11-phenethyl-6,7,8,9,10,11- −0.064 −34.050 hexahydrobenzo[4,5]thieno[3,2-e][1,2,4]triazolo[4,3-a]pyrimidin-1-ium 1020 N-(2-chloro-5-(trifluoromethyl)phenyl)-2-((3-(4-ethoxyphenyl)-4-oxo-3,4- −0.063 −33.897 dihydroquinazolin-2-yl)thio)acetamide 1025 (E)-3-((5-(4-bromophenyl)furan-2-yl)methylene)-1-(naphthalen-2-yl)-5- −0.063 −32.896 phenyl-1H-pyrrol-2(3H)-one 1030 (Z)-5-(4-(benzyloxy)-3-methoxybenzylidene)-2-thioxo-3-(3- −0.063 −31.813 (trifluoromethyl)phenyl)thiazolidin-4-one 1035 N-(5-(2-chloro-5-(trifluoromethyl)benzyl)-4-methylthiazol-2-yl)-5-((p- −0.063 −33.028 tolyloxy)methyl)furan-2-carboxamide 1040 2-((2-(2-chlorobenzyl)-1-oxo-1,2-dihydrobenzo[4,5]thieno[3,2-d]pyrimidin- −0.063 −32.052 3-yl)thio)-N-(m-tolyl)acetamide 1045 2,2′-disulfanediylbis(3-phenylquinazolin-4(3H)-one) −0.063 −32.056 1050 N-(3-(6-(2,5-dimethoxyphenyl)pyridin-2-yl)phenyl)-2,3,4,5,6- −0.063 −31.652 pentafluorobenzamide 1055 2-((4-oxo-3-(4-phenoxyphenyl)-3,4-dihydroquinazolin-2-yl)thio)-N- −0.063 −36.004 (perfluorophenyl)acetamide 1060 N-(5-(2-chloro-5-(trifluoromethyl)benzyl)-4-methylthiazol-2-yl)-5- −0.063 −32.023 (phenoxymethyl)furan-2-carboxamide 1065 6,6′-([1,1′-biphenyl]-2,2′-diylbis(oxy))bis(benzo[de]isochromene-1,3-dione) −0.063 −36.516 1070 5-((5-(2,5-dichlorophenyl)furan-2-yl)methylene)-1,3-diphenyl-2- −0.063 −32.767 thioxodihydropyrimidine-4,6(1H,5H)-dione 1075 2-((2-(9H-fluoren-2-yl)-2-oxoethyl)thio)-6-(4-methoxyphenyl)-4- −0.063 −32.556 (trifluoromethyl)nicotinonitrile 1080 (E)-2-((3-(4-chlorophenyl)-4-oxo-3,4-dihydroquinazolin-2-yl)thio)-N′-((2- −0.063 −32.373 hydroxynaphthalen-1-yl)methylene)acetohydrazide 1085 (E)-5-(5-chloro-2-((3-chlorobenzyl)oxy)benzylidene)-3-(2-chloro-6- −0.063 −32.898 fluorobenzyl)thiazolidine-2,4-dione 1090 (Z)-2-(1-(2-chlorobenzyl)-1H-benzo[d]imidazol-2-yl)-1-(4-fluorophenyl)vinyl −0.063 −31.578 4-fluorobenzoate 1095 ethyl 4-(2-chlorophenyl)-2-(2-(furan-2-yl)quinoline-4- −0.063 −31.618 carboxamido)thiophene-3-carboxylate 1100 9-((2-(4-chlorophenyl)-2-oxoethyl)thio)-8-(m-tolyl)-5,6- −0.063 −33.235 dihydronaphtho[2′,1′:4,5]thieno[2,3-d]pyrimidin-7(8H)-one 1105 2-((1-oxo-2-(pyridin-4-ylmethyl)-1,2,6,7,8,9- −0.063 −33.322 hexahydrobenzo[4,5]thieno[2,3-d]pyrimidin-3-yl)thio)-N-(2- (trifluoromethyl)phenyl)acetamide 1110 3-(5-(benzyloxy)-2,4-dichlorophenyl)-2-(benzylthio)quinazolin-4(3H)-one −0.063 −32.614 1115 dimethyl 5-(1-hydroxy-4-((perfluorophenyl)thio)-2- −0.063 −36.175 naphthamido)isophthalate 1120 N-(2-benzoyl-4-chlorophenyl)-2-((3-cyano-6-phenyl-4- −0.063 −34.591 (trifluoromethyl)pyridin-2-yl)thio)acetamide 1125 4-((1-oxo-3-((2-oxo-2-((2-(trifluoromethyl)phenyl)amino)ethyl)thio)-6,7,8,9- −0.063 −33.280 tetrahydrobenzo[4,5]thieno[2,3-d]pyrimidin-2(1H)-yl)methyl)pyridin-1-ium 1130 (3aS,6aS)-3-(2-naphthoyl)-5-(4-bromophenyl)-1-(4-chlorophenyl)-1,6a- −0.063 −34.965 dihydropyrrolo[3,4-c]pyrazole-4,6(3aH,5H)-dione 1135 (Z)-4-chloro-3-(5-((3-(2-((4-chlorophenyl)amino)-2-oxoethyl)-2,4- −0.063 −32.299 dioxothiazolidin-5-ylidene)methyl)furan-2-yl)benzoic acid 1140 N-(2-benzoyl-4-bromo-9,10-dioxo-9,10-dihydroanthracen-1-yl)-2- −0.063 −34.064 chlorobenzamide 1145 N-((2-(5-chloronaphthalen-1-yl)benzo[d]oxazol-5-yl)carbamothioyl)-5-(3- −0.062 −35.559 nitrophenyl)furan-2-carboxamide 1150 N-(3-((4-chloro-1H-pyrazol-1-yl)methyl)phenyl)-3-phenyl-1- −0.062 −31.345 (trifluoromethyl)-3H-thieno[2,3-c]pyrazole-5-carboxamide 1155 (Z)-5-(3-(2,4-dinitrophenoxy)benzylidene)-2-thioxo-3-(3- −0.062 −34.176 (trifluoromethyl)phenyl)thiazolidin-4-one 1160 (E)-3-(3-((5-(2,5-dichlorophenyl)furan-2-yl)methylene)-2-oxo-5-phenyl-2,3- −0.062 −31.263 dihydro-1H-pyrrol-1-yl)benzoic acid 1165 (Z)-ethyl 3-(((1,3-dioxo-2-(3-(trifluoromethyl)phenyl)-2,3- −0.062 −32.425 dihydroisoquinolin-4(1H)-ylidene)methyl)amino)benzofuran-2-carboxylate 1170 N-(5-chloro-2-phenoxyphenyl)-6-(4-chlorobenzyl)-4-methyl-6H-thieno[2,3- −0.062 −31.659 c]pyrazole-2-carboxamide 1175 2-oxo-2-(10H-phenothiazin-10-yl)ethyl 4′-(trifluoromethyl)-[1,1′-biphenyl]- −0.062 −31.460 2-carboxylate 1180 (Z)-ethyl 4-(5-((2-(4-methylbenzyl)-3,7-dioxo-3H-thiazolo[3,2- −0.062 −31.065 b][1,2,4]triazin-6(7H)-ylidene)methyl)furan-2-yl)benzoate 1185 2-((3-cyano-6-(4-((4-fluorobenzyl)oxy)phenyl)-4-(trifluoromethyl)pyridin-2- −0.062 −34.516 yl)thio)-N-(4-fluorophenyl)acetamide 1190 5-((5-(2-bromo-4-methylphenyl)furan-2-yl)methylene)-1,3-diphenyl-2- −0.062 −33.730 thioxodihydropyrimidine-4,6(1H,5H)-dione 1195 2-(3,5-dichloro-4-(naphthalen-1-ylmethoxy)phenyl)-4,5-diphenyl-1H- −0.062 −32.355 imidazole 1200 N-(4-(2,4-dichlorophenyl)thiazol-2-yl)-2-(3-methoxyphenyl)quinoline-4- −0.062 −31.397 carboxamide 1205 3-((2-(1-(4-fluorophenyl)-2,5-dimethyl-1H-pyrrol-3-yl)-2-oxoethyl)thio)-2,6- −0.062 −35.055 diphenylthieno[2,3-d]pyrimidin-1(2H)-one 1210 3-((2-oxo-2-(4-phenylpiperazin-1-yl)ethyl)thio)-2-(3- −0.062 −34.909 (trifluoromethyl)phenyl)-2,9-dihydro-1H-pyrimido[5,4-13]indol-1-one 1215 3-(4-fluorophenyl)-4-oxo-2-((2-oxo-2-((2,3,4- −0.062 −31.099 trifluorophenyl)amino)ethyl)thio)-3,4-dihydroquinazoline-7-carboxylic acid 1220 2-((3-cyano-4,5-diphenylfuran-2-yl)amino)-2-oxoethyl 1- −0.062 −31.724 chlorobenzo[b]thiophene-2-carboxylate 1225 2-([1,1′-biphenyl]-4-yl)-N-(4-(N-(3,4-dimethylisoxazol-5- −0.062 −35.511 yl)sulfamoyl)phenyl)quinoline-4-carboxamide 1230 (E)-1-(6-chloro-2-methyl-4-phenylquinolin-3-yl)-3-(1,3-diphenyl-1H-pyrazol- −0.062 −32.495 4-yl)prop-2-en-1-one 1235 9-((4-bromobenzyl)thio)-8-(m-tolyl)-5,6- −0.062 −33.660 dihydronaphtho[2′,1′:4,5]thieno[2,3-d]pyrimidin-7(8H)-one 1240 (E)-2-chloro-5-(3-((5-(3,4-dichlorophenyl)furan-2-yl)methylene)-2-oxo-5- −0.062 −33.012 phenyl-2,3-dihydro-1H-pyrrol-1-yl)benzoic acid 1245 2-((3-cyano-6-(4-fluorophenyl)-4-phenylpyridin-2-yl)thio)-N-(3- −0.062 −31.244 (trifluoromethyl)phenyl)acetamide 1250 9-((2-(4-chlorophenyl)-2-oxoethyl)thio)-8-cyclohexyl-5,6- −0.062 −32.060 dihydronaphtho[2′,1′:4,5]thieno[2,3-d]pyrimidin-7(8H)-one 1255 (Z)-2-((3,4-dichlorobenzyl)thio)-3-(2-(2,4,6-trichlorophenyl)hydrazono)-3H- −0.061 −31.713 indole 1260 N-(9,10-dioxo-9,10-dihydroanthracen-1-yl)-2-((3-(4-fluorophenyl)-4-oxo- −0.061 −32.916 3,4-dihydroquinazolin-2-yl)thio)acetamide 1265 N-(3-chloro-4-methylphenyl)-2-((2-(4-ethoxyphenyl)-1-oxo-2,9-dihydro-1H- −0.061 −31.866 pyrimido[5,4-b]indol-3-yl)thio)acetamide 1270 N-(4-chloro-3-(trifluoromethyl)phenyl)-2-((3-(4-chlorophenyl)-4-oxo-3,4- −0.061 −32.165 dihydroquinazolin-2-yl)thio)acetamide 1275 (E)-2-(((5-(3,4-dichlorophenyl)furan-2-yl)methylene)amino)-N-(2- −0.061 −31.219 (trifluoromethyl)phenyl)thiophene-3-carboxamide 1280 (Z)-2-methoxy-4-((4-oxo-2-thioxo-3-(3-(trifluoromethyl)phenyl)thiazolidin- −0.061 −31.563 5-ylidene)methyl)phenyl benzoate 1285 3-(5-((4-chlorophenyl)thio)-2-((2-methylbenzyl)thio)pyrimidine-4- −0.061 −31.882 carboxamido)benzoic acid 1290 (Z)-2-(benzo[d]thiazol-2(1H)-ylidene)-4-((3-benzyl-6-(trifluoromethyl)-3H- −0.061 −31.965 benzo[d]imidazol-2-yl)thio)-3-oxobutanenitrile 1295 (1E,NE)-2-cyano-N-(2-hydroxy-4-(trifluoromethyl)-7H-chromen-7-ylidene)- −0.061 −31.078 2-(4-(2-oxo-2H-chromen-3-yl)thiazol-2-yl)ethenaminium 1300 (S,E)-10-((1-(2-chlorobenzyl)-1H-indol-3-yl)methylene)-7-(thiophen-2-yl)- −0.061 −36.038 7,10-dihydro-5H-benzo[h]thiazolo[2,3-b]quinazolin-9(6H)-one 1305 (E)-5-(5-bromo-2-((2-chlorobenzyl)oxy)benzylidene)-3-(2-chloro-6- −0.061 −34.624 fluorobenzyl)thiazolidine-2,4-dione 1310 (R,E)-10-(2-((2-fluorobenzyl)oxy)benzylidene)-7-(3-fluorophenyl)-7,10- −0.061 −34.316 dihydro-5H-benzo[h]thiazolo[2,3-b]quinazolin-9(6H)-one 1315 2-((3-(4-chlorophenyl)-4-oxo-3,4-dihydroquinazolin-2-yl)thio)-N-(2- −0.061 −33.031 methoxydibenzo[b,d]furan-3-yl)acetamide 1320 (R,Z)-10-(2-((4-chlorobenzyl)oxy)benzylidene)-7-(4-fluorophenyl)-7,10- −0.061 −35.272 dihydro-5H-benzo[h]thiazolo[2,3-b]quinazolin-9(6H)-one 1325 (R,Z)-7-(4-fluorophenyl)-10-(2-(naphthalen-1-ylmethoxy)benzylidene)-7,10- −0.061 −36.208 dihydro-5H-benzo[h]thiazolo[2,3-b]quinazolin-9(6H)-one 1330 (Z)-2-((Z)-5-(2,4-dichlorobenzylidene)-3-(4-fluorophenyl)-4-oxothiazolidin-2- −0.061 −32.700 ylidene)-3-oxo-3-(4-propylphenyl)propanenitrile 1335 (R,Z)-2-(5-((7-(3-methoxyphenyl)-9-oxo-5H-benzo[h]thiazolo[2,3- −0.061 −34.036 b]quinazolin-10(6H,7H,9H)-ylidene)methyl)furan-2-yl)benzoic acid 1340 (S,E)-5-phenyl-2-(3-(4-(5-phenyl-3-(p-tolyl)-4,5-dihydro-1H-pyrazol-1- −0.061 −33.896 yl)styryl)phenyl)oxazole 1345 (E)-methyl 2-chloro-5-(3-((5-(2,5-dichlorophenyl)furan-2-yl)methylene)-2- −0.061 −33.433 oxo-5-phenyl-2,3-dihydro-1H-pyrrol-1-yl)benzoate 1350 2-(4-ethylphenyl)-N-(4-(N-(4-methylpyrimidin-2- −0.061 −31.766 yl)sulfamoyl)phenyl)quinoline-4-carboxamide 1355 N-(3-chloro-4-methoxyphenyl)-3-(2-chloro-5-(trifluoromethyl)phenyl)-4- −0.061 −32.768 oxo-2-thioxo-1,2,3,4-tetrahydroquinazoline-7-carboxamide 1360 5-(5-bromo-2-hydroxybenzoyl)-N,1-bis(2-fluorophenyl)-2-oxo-1,2- −0.061 −31.773 dihydropyridine-3-carboxamide 1365 4-((5-hydroxy-2-oxo-1-(3-(trifluoromethyl)benzyl)-2,3-dihydro-1H-imidazol- −0.061 −31.643 4-yl)methylene)-1-phenyl-3-(thiophen-2-ylmethyl)-1H-pyrazol-5(4H)-one 1370 (Z)-3-(5-((1-(3,4-dichlorobenzyl)-1H-indol-3-yl)methylene)-4-oxo-2- −0.061 −32.583 thioxothiazolidin-3-yl)benzoic acid 1375 1-(4-bromophenyl)-6-(4-((2-fluorobenzyl)oxy)phenyl)-3-methyl-4- −0.060 −33.631 (trifluoromethyl)-1H-pyrazolo[3,4-b]pyridine 1380 N-(2-chloro-5-(trifluoromethyl)phenyl)-2-((1-oxo-2,6-diphenyl-1,2- −0.060 −34.554 dihydrothieno[2,3-d]pyrimidin-3-yl)thio)acetamide 1385 (S)-2-((4-chloro-2-(trifluoromethyl)phenyl)amino)-2-oxo-1-phenylethyl 1- −0.060 −31.646 chlorobenzo[b]thiophene-2-carboxylate 1390 N-(4-chlorophenyl)-2-((3-cyano-6-(4-((4-fluorobenzyl)oxy)phenyl)-4- −0.060 −34.501 (trifluoromethyl)pyridin-2-yl)thio)acetamide 1395 N,N′-(1,2-phenylene)bis(1-chloro-5-methoxybenzo[b]thiophene-2- −0.060 −33.603 carboxamide) 1400 N-(2,5-dichlorophenyl)-2-((4-oxo-3-(4-phenoxyphenyl)-3,4- −0.060 −33.030 dihydroquinazolin-2-yl)thio)acetamide 1405 2-((3-(4-bromophenyl)-4-oxo-3,4-dihydroquinazolin-2-yl)thio)-N-(3-chloro- −0.060 −31.240 4-fluorophenyl)acetamide 1410 N-(3,5-dimethylphenyl)-2-((1-oxo-2-(3-(trifluoromethyl)phenyl)-2,9- −0.060 −31.441 dihydro-1H-pyrimido[5,4-b]indol-3-yl)thio)acetamide 1415 N-(2-bromo-4,6-difluorophenyl)-2-((3-(4-methoxyphenyl)-4-oxo-3,4- −0.060 −32.023 dihydroquinazolin-2-yl)thio)acetamide 1420 N-(4-(2,4-dichlorophenyl)thiazol-2-yl)-2-(4-ethoxyphenyl)quinoline-4- −0.060 −31.299 carboxamide 1425 (Z)-3-(5-((1-(2,4-dichlorobenzyl)-1H-indol-3-yl)methylene)-4-oxo-2- −0.060 −32.346 thioxothiazolidin-3-yl)benzoic acid 1430 N2,N7-bis(4-chlorophenyl)-9-oxo-9H-fluorene-2,7-disulfonamide −0.060 −33.572 1435 2-((3-(4-bromophenyl)-4-oxo-3,4-dihydroquinazolin-2-yl)thio)-N-(4-chloro- −0.060 −34.099 3-(trifluoromethyl)phenyl)acetamide 1440 (Z)-2-(3-methoxybenzylidene)-3-oxo-2,3-dihydrobenzofuran-6-yl 5- −0.060 −31.066 methoxy-2-phenylbenzofuran-3-carboxylate 1445 N-(3-chloro-1,4-dioxo-1,4-dihydronaphthalen-2-yl)-4-(2-((1,2-dioxo-1,2- −0.060 −33.026 dihydronaphthalen-4-yl)amino)phenyl)-2-hydroxy-4-oxobut-2-enamide 1450 5-butoxy-N-(2-(3-chloro-4-methoxybenzoyl)benzofuran-3-yl)-2- −0.060 −31.784 methylbenzofuran-3-carboxamide 1455 2-([1,1′-biphenyl]-4-yl)-N-(3-(N-methyl-N- −0.060 −34.002 phenylsulfamoyl)phenyl)quinoline-4-carboxamide 1460 (E)-3-(3,4-bis(benzyloxy)phenyl)-2-(4-(3-nitrophenyl)thiazol-2- −0.060 −32.527 yl)acrylonitrile 1465 bis(2-(naphthalen-2-yl)-2-oxoethyl) [1,1′-biphenyl]-2,2′-dicarboxylate −0.060 −34.456 1470 (Z)-5-((4-bromo-5-((4-chlorophenyl)thio)furan-2-yl)methylene)-1-(4- −0.060 −32.007 fluorophenyl)-2-thioxodihydropyrimidine-4,6(1H,5H)-dione 1475 5,6,11,12-tetraphenyltetracene −0.059 −31.681 1480 (S)-2-(3-(2-(5-(4-fluorophenyl)-3-(thiophen-2-yl)-4,5-dihydro-1H-pyrazol-1- −0.059 −32.707 yl)thiazol-4-yl)phenyl)isoindoline-1,3-dione 1485 N-(1-chloro-9,10-dioxo-9,10-dihydroanthracen-2-yl)-2-(4- −0.059 −31.761 fluorophenylsulfonamido)benzamide 1490 (Z)-3-(3,4-difluorobenzyl)-5-(4-(4-(furan-2-carbonyl)piperazine-1- −0.059 −31.897 carbonyl)benzylidene)thiazolidine-2,4-dione 1495 (S,Z)-5-(4-chlorobenzylidene)-2-(5-(4-methoxyphenyl)-3-(naphthalen-2-yl)- −0.059 −31.078 4,5-dihydro-1H-pyrazol-1-yl)thiazol-4(5H)-one 1500 3-(5-((E)-((Z)-2-((4-bromo-2-chlorophenyl)imino)-4-oxothiazolidin-5- −0.059 −31.850 ylidene)methyl)furan-2-yl)-4-chlorobenzoic acid 1505 1,3-bis((6-phenylthieno[2,3-d]pyrimidin-1-yl)oxy)benzene −0.059 −31.440 1510 N-(4-fluorobenzyl)-11-oxo-10-(4-(trifluoromethyl)benzyl)-10,11- −0.059 −31.778 dihydrodibenzo[b,f][1,4]thiazepine-8-carboxamide 1515 2,2′-(quinoxaline-2,3-diylbis(4,1-phenylene))bis(1-(4-fluorophenyl)ethane- −0.059 −34.476 1,2-dione) 1520 (E)-3-(1-(2-chloro-5-(trifluoromethyl)phenyl)-2,5-dimethyl-1H-pyrrol-3-yl)-2- −0.059 −31.289 (4-oxo-1,4,5,6,7,8-hexahydrobenzo[4,5]thieno[2,3-d]pyrimidin-2- yl)acrylonitrile 1525 (Z) - N-(2-(2-chloro-4,5-difluorophenyl)benzo[d]oxazol-5-yl)carbamothioyl-5- −0.059 −34.163 (2,5-dichlorophenyl)furan-2-carbimidate 1530 N-(6-methylbenzo[d]thiazol-2-yl)-2-((2-(4-nitrophenyl)-1-oxo-2,9-dihydro- −0.059 −32.067 1H-pyrimido[5,4-b]indol-3-yl)thio)acetamide 1535 N-(3-cyano-4-(2,4-dichlorophenyl)-5-methylthiophen-2-yl)-2-(3- −0.059 −32.971 ethoxyphenyl)quinoline-4-carboxamide 1540 (Z)-2-(2-(3-(3-((2-chlorobenzyl)oxy)phenyl)-1-phenyl-1H-pyrazol-4-yl)-1- −0.059 −32.753 cyanovinyl)quinazolin-4-olate 1545 (E)-2-(4-((3,4-dichlorobenzyl)oxy)styryl)-3-(3- −0.059 −33.473 (trifluoromethyl)phenyl)quinazolin-4(3H)-one 1550 N-(5-benzyl-3-carbamoylthiophen-2-yl)-5-(5-chlorothiophen-2-yl)-7- −0.059 −33.133 (trifluoromethyl)pyrazolo[1,5-a]pyrimidine-2-carboxamide 1555 4,4′-oxybis(N-(4-(pyridin-2-yl)thiazol-2-yl)benzamide) −0.059 −33.945 1560 (S)-3,3-bis(4-chlorophenyl)-3′,5′-diphenyl-3H,3′H-spiro[benzo[c]thiophene- −0.059 −34.203 1,2′-[1,3,4]thiadiazole] 1565 N-(4-(6-methylbenzo[d]thiazol-2-yl)phenyl)-2-((4-oxo-3-(p-tolyl)-3,4- −0.059 −32.238 dihydroquinazolin-2-yl)thio)acetamide 1570 (E)-2-((6-((2-chloro-6-fluorobenzylidene)amino)benzo[d]thiazol-2-yl)thio)-N- −0.059 −32.060 (dibenzo[b,d]furan-3-yl)acetamide 1575 2-((2-(1-(4-chlorophenethyl)-2,5-dimethyl-1H-pyrrol-3-yl)-2-oxoethyl)thio)- −0.059 −32.021 3-(4-fluorophenyl)quinazolin-4(3H)-one 1580 N-(4-(benzyloxy)phenyl)-2-((7-chloro-4-oxo-3-(p-tolyl)-3,4- −0.059 −31.771 dihydroquinazolin-2-yl)thio)acetamide 1585 3-((2-(4-bromophenyl)-2-oxoethyl)thio)-10-oxo-11-phenethyl-6,7,8,9,10,11- −0.059 −34.020 hexahydrobenzo[4,5]thieno[3,2-e][1,2,4]triazolo[4,3-a]pyrimidin-1-ium 1590 5-(5-chlorothiophen-2-yl)-N-(3-(cyclohexylcarbamoyl)-4-ethyl-5- −0.059 −34.905 methylthiophen-2-yl)-7-(trifluoromethyl)pyrazolo[1,5-a]pyrimidine-2- carboxamide 1595 3-((E)-1-((Z)-((4E,5Z)-3-phenyl-4,5-bis(phenylimino)thiazolidin-2- −0.058 −31.674 ylidene)hydrazono)ethyl)-2H-chromen-2-one 1600 2,2′,3,3′-tetraphenyl-7,7′-biquinoxaline −0.058 −32.880 1605 (E)-5-(5-bromo-2-((4-chlorobenzyl)oxy)benzylidene)-3-(2,6- −0.058 −34.072 dichlorobenzyl)thiazolidine-2,4-dione 1610 1-(4-bromophenyl)-6-(4-((2-chlorobenzyl)oxy)phenyl)-3-methyl-4- −0.058 −33.400 (trifluoromethyl)-1H-pyrazolo[3,4-b]pyridine 1615 (E) - N-(5-(4-bromobenzyl)thiazol-2-yl)-2-cyano-3-(5-(3-nitrophenyl)furan-2- −0.058 −31.195 yl)acrylamide 1620 6-(5-chloro-3-(9-chloro-5H-chromeno[4,3-b]pyridin-3-yl)benzofuran-2- −0.058 −32.461 carbonyl)-4-methyl-2H-benzo[b][1,4]oxazin-3(4H)-one 1625 N-(4-chloro-3-(trifluoromethyl)phenyl)-2-((2-(4-methoxyphenyl)-1-oxo- −0.058 −33.749 1,2,6,7,8,9-hexahydrobenzo[4,5]thieno[2,3-d]pyrimidin-3-yl)thio)acetamide 1630 N1,N3-bis(3-(benzo[d]oxazol-2-yl)phenyl)isophthalamide −0.058 −32.003 1635 (E)-3-((5-(4-bromophenyl)furan-2-yl)methylene)-1-(2,4-dichlorophenyl)-5- −0.058 −31.219 phenyl-1H-pyrrol-2(3H)-one 1640 2-((3-(4-bromophenyl)-4-oxo-3,4-dihydroquinazolin-2-yl)thio)-N-(3,5- −0.058 −31.067 dichlorophenyl)acetamide 1645 N-(5-(4-chlorophenyl)-1,2,4-thiadiazol-3-yl)-2-((3-cyano-4-(4- −0.058 −33.072 methoxyphenyl)-6-phenylpyridin-2-yl)thio)acetamide 1650 3-(5-((4-(4-carboxyphenoxy)phenyl)sulfonyl)-1,3-dioxoisoindolin-2- −0.058 −31.387 yl)benzoic acid 1655 (Z) - N-(2-((5-ethyl-4-(4-phenoxyphenyl)thiazol-2-yl)carbamoyl)-3H- −0.058 −34.454 benzo[f]chromen-3-ylidene)benzenaminium 1660 N-(3-(N-(2-chlorophenyl)sulfamoyl)phenyl)-2-(4-methoxyphenyl)quinoline- −0.058 −31.504 4-carboxamide 1665 (E)-2-((2-(1-(4-chlorobenzyl)-1H-pyrazole-3- −0.058 −31.789 carbonyl)hydrazono)methyl)phenyl 1-chlorobenzo[b]thiophene-2- carboxylate 1670 N-(3-(N-(2-chlorophenyl)sulfamoyl)-4-methylphenyl)-2-(4- −0.058 −32.262 methoxyphenyl)quinoline-4-carboxamide 1675 (R,Z) - N-(2-chlorophenyl)-2-cyano-2-(5-(2,5-dichlorobenzyl)-4-oxo-3-(p- −0.058 −31.352 tolyl)thiazolidin-2-ylidene)acetamide 1680 N-(2-methoxy-4-(3-(5-(3-nitrophenyl)furan-2- −0.058 −32.119 carbonyl)thioureido)phenyl)benzofuran-2-carboxamide 1685 di(naphthalen-2-yl) 9H-fluorene-2,7-disulfonate −0.058 −33.372 1690 (Z)-5-chloro-N-(4-(1-(2-(8-chloro-2-phenylquinoline-4- −0.058 −32.246 carbonyl)hydrazono)ethyl)phenyl)thiophene-2-carboxamide 1695 N-(2-chloro-5-(trifluoromethyl)phenyl)-2-((1-oxo-2-(p-tolyl)-1,2,6,7,8,9- −0.058 −32.495 hexahydrobenzo[4,5]thieno[2,3-d]pyrimidin-3-yl)thio)acetamide 1700 (S,E)-10-(3,5-dichloro-2-hydroxybenzylidene)-7-(2,4-dichlorophenyl)-7,10- −0.058 −33.002 dihydro-5H-benzo[h]thiazolo[2,3-b]quinazolin-9(6H)-one 1705 (S)-4-(5-(4-(dimethylamino)phenyl)-1-(4-nitrophenyl)-4,5-dihydro-1H- −0.057 −33.363 pyrazol-3-yl)-7H-benzo[de]benzo[4,5]imidazo[2,1-a]isoquinolin-7-one 1710 4,4′-oxybis(N-(5-fluorobenzo[d]thiazol-2-yl)benzamide) −0.057 −32.065 1715 2-((2-(4-chlorophenyl)-1-oxo-1,2,6,7,8,9-hexahydrobenzo[4,5]thieno[2,3- −0.057 −31.544 d]pyrimidin-3-yl)thio)-N-(2-(trifluoromethyl)phenyl)acetamide 1720 2-((3-cyano-6-(4-methoxyphenyl)-4-(trifluoromethyl)pyridin-2-yl)thio)-N- −0.057 −31.341 (2,4,5-trichlorophenyl)acetamide 1725 (S,Z)-10-((5-(4-chloro-2-nitrophenyl)furan-2-yl)methylene)-7-(thiophen-2- −0.057 −32.761 yl)-7,10-dihydro-5H-benzo[h]thiazolo[2,3-b]quinazolin-9(6H)-one 1730 (R,E)-10-(2-((2-chlorobenzyl)oxy)benzylidene)-7-(4-chlorophenyl)-7,10- −0.057 −34.059 dihydro-5H-benzo[h]thiazolo[2,3-b]quinazolin-9(6H)-one 1735 N-(2-(3-((2-(benzo[d]thiazol-2-ylamino)-2-oxoethyl)thio)-1H-indol-1- −0.057 −31.683 yl)ethyl)-3-(trifluoromethyl)benzamide 1740 5-bromo-2-(5-(5-chlorothiophen-2-yl)-7-(trifluoromethyl)pyrazolo[1,5- −0.057 −31.086 a]pyrimidine-2-carboxamido)benzoic acid 1745 N4,N4′-bis(2-chlorophenyl)-[3,3′-biquinoline]−4,4′-dicarboxamide −0.057 −32.139 1750 (R)-5-(3-(benzyloxy)phenyl)-4-(2,3-dihydrobenzo[b][1,4]dioxine-7-carbonyl)- −0.057 −33.613 3-hydroxy-1-(5-methylbenzo[d]thiazol-2-yl)-1H-pyrrol-2(5H)-one 1755 3-(2-((3-chloro-4-((1-chloronaphthalen-2-yl)oxy)phenyl)amino)thiazol-4-yl)- −0.057 −31.994 8-methoxy-2H-chromen-2-one 1760 N2,N6-bis(2-(benzo[d]thiazol-2-yl)phenyl)pyridine-2,6-dicarboxamide −0.057 −33.245 1765 3-((2-(2,5-dimethyl-1-(p-tolyl)-1H-pyrrol-3-yl)-2-oxoethyl)thio)-2,6- −0.057 −31.964 diphenylthieno[2,3-d]pyrimidin-1(2H)-one 1770 (Z)-2-(2-(3-(3-((4-chlorobenzyl)oxy)phenyl)-1-phenyl-1H-pyrazol-4-yl)-1- −0.057 −31.539 cyanovinyl)quinazolin-4-olate 1775 N1,N3-di(naphthalen-2-yl)-N1,N3-diphenylisophthalamide −0.057 −32.254 1780 (N,N′E,N,N′Z) - N,N′-((Z)-2-((E)-(anthracen-9-ylmethylene)hydrazono)-3- −0.057 −31.682 phenylthiazolidine-4,5-diylidene)dianiline 1785 (S)-(2-((difluoromethyl)thio)phenyl)(6-methoxy-1-(5-(2- −0.057 −33.843 (trifluoromethyl)phenyl)furan-2-yl)-3,4-dihydro-1H-pyrido[3,4-b]indol- 2(9H)-yl)methanone 1790 N2,N5-bis(1-ethyl-2-oxo-1,2-dihydrobenzo[cd]indol-6-yl)thiophene-2,5- −0.057 −31.688 dicarboxamide 1795 (R)-1-(3,4-dimethoxyphenyl)-3-((1-oxo-2-(m-tolyl)-1,2,6,7,8,9- −0.056 −31.706 hexahydrobenzo[4,5]thieno[2,3-d]pyrimidin-3-yl)thio)pyrrolidine-2,5-dione 1800 (9H-fluorene-9,9-diyl)bis(4,1-phenylene) bis(2-fluorobenzoate) −0.056 −33.529 1805 11-chloro-N-(1-(2,4-dichlorobenzyl)-1H-pyrazol-4-yl)-7-(trifluoromethyl)- −0.056 −33.355 5,6-dihydrobenzo[h]pyrazolo[5,1-b]quinazoline-10-carboxamide 1810 (8S,8aR,11aS,11bS)-10-(2,5-dichlorophenyl)-9,11-dioxo-N-(3- −0.056 −32.180 (trifluoromethyl)phenyl)-8a,9,10,11,11a,11b-hexahydro-8H- pyrrolo[3′,4′:3,4]pyrrolo[2,1-a]isoquinoline-8-carboxamide 1815 (R)-4-(benzofuran-2-carbonyl)-3-hydroxy-1-(5-((naphthalen-1- −0.056 −32.227 ylmethyl)thio)-1,3,4-thiadiazol-2-yl)-5-phenyl-1H-pyrrol-2(5H)-one 1820 (R,E)-10-((5-(2,3-dichlorophenyl)furan-2-yl)methylene)-7-(4-fluorophenyl)- −0.056 −32.123 7,10-dihydro-5H-benzo[h]thiazolo[2,3-b]quinazolin-9(6H)-one 1825 2,2′-(2-(1,2-diphenyl-1H-indol-3-yl)ethene-1,1-diyl)bis(benzo[d]thiazole) −0.056 −31.408 1830 2-(3-bromophenyl)-N-(4-(N-(4-methylpyrimidin-2- −0.056 −32.069 yl)sulfamoyl)phenyl)quinoline-4-carboxamide 1835 6,6′-(carbonylbis(4,1-phenylene))bis(5-phenylpyrazine-2,3-dicarbonitrile) −0.056 −32.953 1840 (Z)-3-(4-benzylpyridin-1-ium-1-yl)-2-(((4-bromophenyl)sulfonyl)imino)-1- −0.056 −31.191 oxo-1,2-dihydronaphthalen-4-olate 1845 (R)-4-(benzofuran-2-carbonyl)-3-hydroxy-1-(5-((naphthalen-1- −0.056 −32.745 ylmethyl)thio)-1,3,4-thiadiazol-2-yl)-5-(p-tolyl)-1H-pyrrol-2(5H)-one 1850 2-phenyl-N-(2-(4-(2-phenylquinoline-4-carbonyl)piperazin-1- −0.056 −32.918 yl)ethyl)quinoline-4-carboxamide 1855 2-(4-chlorophenyl)-2-oxoethyl 2-(4-((3aR,7aR)-1,3-dioxohexahydro-1H- −0.056 −31.478 isoindol-2(3H)-yl)phenyl)-6-methylquinoline-4-carboxylate 1860 (Z)-2-(2,4-dichlorophenyl)-5-((3-(4-ethoxy-3-methylphenyl)-1-phenyl-1H- −0.055 −31.849 pyrazol-4-yl)methylene)thiazolo[3,2-b][1,2,4]triazol-6(5H)-one 1865 (Z)-3-oxo-2-(3,4,5-trimethoxybenzylidene)-2,3-dihydrobenzofuran-6-yl 5- −0.055 −32.016 methoxy-2-phenylbenzofuran-3-carboxylate 1870 (Z)-5-(5-bromo-2-((3,4-dichlorobenzyl)oxy)benzylidene)-3-(4-fluorobenzyl)- −0.055 −32.223 2-thioxothiazolidin-4-one 1875 (1E,NE)-2-(4-(6-bromo-2-oxo-2H-chromen-3-yl)thiazol-2-yl)-2-cyano-N-(2- −0.055 −32.406 hydroxy-4-(trifluoromethyl)-7H-chromen-7-ylidene)ethenaminium 1880 (Z)-2-(2,4-dichlorophenyl)-5-((1-phenyl-3-(4-propoxyphenyl)-1H-pyrazol-4- −0.055 −31.645 yl)methylene)thiazolo[3,2-b][1,2,4]triazol-6(5H)-one 1885 10-(3-(4-(4-(4-chlorophenyl)thiazol-2-yl)piperazin-1-yl)propyl)-2- −0.055 −32.346 (trifluoromethyl)-10H-phenothiazine 1890 (S)-2-((3-allyl-4-oxo-3,4,5,6,7,8-hexahydrobenzo[4,5]thieno[2,3- −0.055 −32.407 d]pyrimidin-2-yl)thio)-N-(2-chloro-5-(trifluoromethyl)phenyl)-2- phenylacetamide 1895 (R,E)-2-chloro-4-(5-((7-(4-fluorophenyl)-9-oxo-5H-benzo[h]thiazolo[2,3- −0.055 −31.928 b]quinazolin-10(6H,7H,9H)-ylidene)methyl)furan-2-yl)benzoic acid 1900 (E)-4-((2-(1-naphthoyl)hydrazono)methyl)-1,3-phenylene bis(4- −0.055 −31.973 chlorobenzoate) 1905 2-(5-(2,5-dichlorophenyl)furan-2-yl)-N-(5-(4-fluorobenzyl)thiazol-2- −0.055 −31.440 yl)quinoline-4-carboxamide 1910 N-(9-ethyl-9H-carbazol-3-yl)-1,3-dioxo-2-(3-(trifluoromethyl)phenyl)-2,3- −0.055 −31.523 dihydro-1H-benzo[de]isoquinoline-6-carboxamide 1915 (Z)-2-(2,4-dichlorophenyl)-5-((3-(4-isopropoxy-3-methylphenyl)-1-phenyl- −0.054 −32.061 1H-pyrazol-4-yl)methylene)thiazolo[3,2-b][1,2,4]triazol-6(5H)-one 1920 N-benzyl-N-(2-(2-(naphthalen-2-yl)imidazo[2,1-b]thiazol-5-yl)ethyl)-3- −0.054 −32.201 (trifluoromethyl)benzenesulfonamide 1925 3-((2-(2,5-dimethyl-1-(3-(trifluoromethyl)phenyl)-1H-pyrrol-3-yl)-2- −0.054 −32.268 oxoethyl)thio)-2-phenyl-6,7,8,9-tetrahydrobenzo[4,5]thieno[2,3- d]pyrimidin-1(2H)-one 1930 (S)-4-(benzofuran-2-carbonyl)-3-hydroxy-5-(5-methylfuran-2-yl)-1-(5- −0.054 −31.424 ((naphthalen-1-ylmethyl)thio)-1,3,4-thiadiazol-2-yl)-1H-pyrrol-2(5H)-one 1935 N-(3-(3,4-dichlorophenylsulfonamido)-4-methoxyphenyl)-2- −0.054 −31.342 phenylquinoline-4-carboxamide 1940 N2,N7-di(naphthalen-1-yl)-9-oxo-9H-fluorene-2,7-disulfonamide −0.054 −31.920 1945 3-((4-chlorophenyl)thio)-N-(4-((1,3-dioxo-2-(2- −0.054 −32.177 (trifluoromethyl)phenyl)isoindolin-6-yl)oxy)phenyl)propanamide 1950 (Z)-5-((4-bromo-5-((4-chlorophenyl)thio)furan-2-yl)methylene)-3-(2,3- −0.054 −31.592 dichlorophenyl)-6-hydroxy-2-thioxo-2,3-dihydropyrimidin-4(5H)-one 1955 N-((4-(benzo[d]thiazol-2-yl)-3-chlorophenyl)carbamothioyl)-4-((4- −0.054 −31.867 chlorobenzyl)oxy)-3-methoxybenzamide 1960 (Z)-4-(((3-(3-fluorobenzoyl)-4,5,6,7-tetrahydrobenzo[b]thiophen-2- −0.054 −31.612 yl)amino)methylene)-2-(3-(trifluoromethyl)phenyl)isoquinoline-1,3(2H,4H)- dione 1965 sulfonylbis(4,1-phenylene) bis(2,4-dichlorobenzoate) −0.053 −31.864 1970 (E)-3-((5-(2,5-dichlorophenyl)furan-2-yl)methylene)-1-(3-iodophenyl)-5- −0.053 −31.161 phenyl-1H-pyrrol-2(3H)-one 1975 (Z)-4-methyl-N-(2-((5-methyl-4-(4-phenoxyphenyl)thiazol-2-yl)carbamoyl)- −0.053 −31.587 3H-benzo[f]chromen-3-ylidene)benzenaminium 1980 (S,Z)-10-(3-bromo-4-hydroxy-5-nitrobenzylidene)-7-(2-chlorophenyl)-7,10- −0.053 −31.495 dihydro-5H-benzo[h]thiazolo[2,3-b]quinazolin-9(6H)-one 1985 (E) - N-(2-(2-(anthracen-9-ylmethylene)hydrazinyl)-2-oxoethyl)-N-(4-chloro- −0.053 −31.586 3-(trifluoromethyl)phenyl)benzenesulfonamide 1990 N-(2-benzoyl-4-bromophenyl)-5-(4-methoxyphenyl)-7- −0.053 −31.308 (trifluoromethyl)pyrazolo[1,5-a]pyrimidine-2-carboxamide 1995 6-bromo-N-(4-(2,4-dichlorophenyl)-5-methylthiazol-2-yl)-2-(4- −0.052 −31.409 methoxyphenyl)quinoline-4-carboxamide 2000 (Z)-3-(furan-2-ylmethyl)-5-((3-(4-(naphthalen-1-ylmethoxy)phenyl)-1- −0.052 −31.069 phenyl-1H-pyrazol-4-yl)methylene)-2-thioxothiazolidin-4-one

The entire disclosures of all applications, patents and publications, cited herein and of corresponding European Application No. 13/172575.6, filed Jun. 18, 2013 are incorporated by reference herein. 

1-12. (canceled)
 13. A method for treating cancer, comprising administering to a subject in need thereof an effective amount of a DYRK1B inhibitor, wherein said cancer and/or cells of said cancer do not respond to Smoothened inhibitor therapy, or said cancer is not responsive to inhibition of the G protein-coupled receptor Smoothened, or in said cancer and/or in cells of said cancer the G protein-coupled receptor Smoothened is not responsive to inhibition by Smoothened inhibitors.
 14. The method of claim 13, wherein said cancer and/or cells of said cancer do not respond to Smoothened inhibitor therapy.
 15. The method of claim 13, wherein said cancer is not responsive to inhibition of the G protein-coupled receptor Smoothened.
 16. The method of claim 13, wherein in said cancer and/or in cells of said cancer the G protein-coupled receptor Smoothened is not responsive to inhibition by Smoothened inhibitors.
 17. The method of claim 13, wherein said cancer is selected from the group consisting of cancer of the breast, esophagus, gastrointestinal tract, gastro-intestinal stromal tumors, pancreas, prostate, biliary tract, bladder, basal cell carcinoma, medulloblastoma, rhabdomyosarcoma, glioma, small-cell lung cancer, oral squamous cell carcinoma, melanoma, colorectal cancer, non-small cell lung cancer, osteosarcoma, glioblastoma, chronic lymphacytic leukemia, chronic myeloid leukemia, multiple myeloma, acute myeloid leukemia, ovarian cancer, meningioma, Non-NF2 Meningiomas, and liver cancer.
 18. The method of claim 13, wherein in said cancer and/or in cells of said cancer the hedgehog signaling pathway is activated.
 19. The method of claim 18, wherein in said cancer and/or in cells of said cancer the activation of the hedgehog signaling pathway is independent of signaling by the G protein-coupled receptor Smoothened.
 20. The method of claim 13, wherein said DYRK1B inhibitor is a small molecule.
 21. The method of claim 13, wherein said DYRK1B inhibitor is a compound of formula (I):


22. The method of claim 13, wherein said patient is a human.
 23. The method of claim 13, wherein cancer stem cells are inhibited in said cancer. 